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Cytotoxicity of Acetoxyscirpendiol from Paecilomyces tenuipes  

한희창 (서울시립대학교 생명과학과 분자면역학실)
김미정 (서울시립대학교 생명과학과 분자면역학실)
김종수 (서울시립대학교 생명과학과 분자면역학실)
김하원 (서울시립대학교 생명과학과 분자면역학실)
Publication Information
YAKHAK HOEJI / v.48, no.2, 2004 , pp. 153-158 More about this Journal
Abstract
Paecilomyces tenuipes is one of the famous Chinese medicinal entomopathogenic fungi that parasite in the lavae of silkworm. A cytotoxic compound, 4$\beta$-acetoxyscirpendiol (ASD) was isolated from a methanolic extracts of Paecilomyces tenuipes. The ASD compound belongs to scirpenol subfamily of trichothecene mycotoxin. In a continuation of the elucidation of the mechanism of ASD, we report here the evidences of induction of apoptosis by ASD in human Jurkat T cell line. In MTT reduction assay for monitoring cell viability, ASD showed strong toxicity. The 50 percent inhibitory concentrations of ASD against human T lymphoid Jurkat cell was 59.5 ng/$m\ell$. Phosphatidylserine externalization was increased by ASD at 3 and 6 hrs when compared with that of 6 hrs in the cell line showing in a time-dependent manner. When whole lysates of cells treated with ASD were subjected to western blot assay, 113 kDa poly(ADP-ribose) polymerase (PARP) was significantly cleaved to 89 kDa fragment. Time-dependent DNA fragmentation was also observed when Jurkat T cells were treated with ASD at 100 ng/$m\ell$ for 6 hrs and 18 hrs at the ratios of 8.5% and 15.0%, respectively. From these data, Jurkat T lymphocytes treated with ASD from Paecilomyces tenuipes underwent typical cascades of apoptotic cell death.
Keywords
Paecilomyces tenuipes; 4$\beta$-acetoxyscirpendiol; apoptosis; PARP; phosphatidylserine;
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