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Effect of NDM Hair Tonic on Hair Growth Promotion in C57BL6 Mice  

남상윤 (충북대학교 수의과대학 및 동물의학연구소)
문준환 (충북대학교 수의과대학 및 동물의학연구소)
윤영원 (충북대학교 수의과대학 및 동물의학연구소)
백인정 (충북대학교 수의과대학 및 동물의학연구소)
연정민 (충북대학교 수의과대학 및 동물의학연구소)
류광철 ((주)다모코스메틱)
이범준 (충북대학교 수의과대학 및 동물의학연구소)
Publication Information
YAKHAK HOEJI / v.48, no.2, 2004 , pp. 122-129 More about this Journal
Abstract
NDM hair tonic is composed of several plant extracts which are known to be used in oriental medicine. This study was carried out to investigate the effect of NDM hair tonic on hair regrowth in an alopecia model of C57BL6 mice. Hair of six-weeks old mice were trimmed by electric clippers and electric shavers, so as not to damage the skin. The next day, mice without visible scraches were selected, randomized and separated in groups of 6 mice. There were four experimental groups including saline (negative control), 50% ethanol (vehicle control), 3% minoxidil (MXD), and NDM tonic. The test compounds were topically treated with 0.15 $m\ell$ per mouse per day for 2 weeks. The hair regrowth was determined photographically and histologically and the quantity of endocrine factors, IGF-1 and TGF-$\beta$, in the skin of mice was measured by PCR. No clinical signs were found in all animals. The topical treatment of NDM tonic for 2 weeks to dorsal skin accelerated hair regrowth faster than either the controls or MXD treatment. The NDM tonic treatment also promoted hair follicle development and elongation as compared with the controls or MXD treatment. Both NDM tonic and MXD treatment significantly increased the expression of IGF-1 and TGF-$\beta$ in the skin of C57BL6 mice as compared with the controls (p<0.05). These results suggest that NDM tonic has a hair growth activity and be useful for the treatment of baldness or alopecia.
Keywords
alopecia; hair growth; IGF-1; NDM hair tonic; TGF-$\beta$;
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