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Analysis of Genetic Diversity of Bifidobacterium spp. Isolated from Korean Adults Fecal Samples  

배학균 (삼육대학교 보건복지대학원)
김선옥 (삼육식품㈜)
박종선 (삼육대학교 약학과)
강병용 (삼육대학교 생명과학연구소)
최성숙 (삼육의명대학 식품과학과)
강진양 (삼육대학교 약학과)
하남주 (삼육대학교 약학과)
Publication Information
YAKHAK HOEJI / v.48, no.1, 2004 , pp. 20-26 More about this Journal
Abstract
Twelve strains of Bifidobacterium spp. were isolated from the feces of healthy Korean 20∼30 years. The identification of genera from isolates were performed by the microscopic observation and fructose-6-phosphate phosphoketolase (F6PPK) activity which is the key enzyme to distinguish the Bifidobacterium spp. from other anaerobic bacteria. To determine the antibacterial resistance patterns, minimum inhibitory concentration (MIC) of several antibiotics (including anti-tuberculosis agents) was determined. Five of the isolate!, showed the high degree of resistance to vancomycin. To investigate the genetic diversity between isolates and type strain of Bifidobacterium spp. from KCTC, we peformed the RAPD-fingerprinting. Using a total set of four primers, it is possible to distinguish the isolates and Bifidobacterium spp. from KCTC. Thus, Bifidobacterium strains isolated from our samples may be a new species or strains of Bifidobacteriurn genera, and have the potential as a probiotics.
Keywords
Bifidobacterium; F6PPK; fingerprinting and probiotics;
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Times Cited By KSCI : 8  (Citation Analysis)
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