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Inhibitory Effect of Eurya emarginata on the Production of Pro-inflammatory Cytokines in Murine Macrophage RAW264.7  

박수영 (제주대학교 의과대학 약리학교실)
이혜자 (제주대학교 의과대학 약리학교실)
현은아 (제주대학교 자연과학대학 생명과학과)
문지영 (제주대학교 자연과학대학 화학과)
앙홍철 (제주대학교 자연과학대학 화학과)
이남호 (제주대학교 자연과학대학 화학과)
김세재 (제주대학교 자연과학대학 생명과학)
강희경 (제주대학교 의과대학 약리학교실)
유은숙 (제주대학교 의과대학 약리학교실)
Publication Information
YAKHAK HOEJI / v.47, no.5, 2003 , pp. 311-318 More about this Journal
Abstract
Eurya emarginata (Thunb.) Makino (Theaceae) is distributed in coastal areas of island. The leaves of Eurya are used in the traditional medicine of the coastal areas of jeju island with the aim of diuresis or to treat ulcers. Nevertheless, there are few reports on the biological activity and constituents of E. emarginata. In this study, we investigated the pharmacological activity of the solvent extracts of E. emarginata on the several inflammatory markers (TNF-$\alpha$, IL-1$\beta$, IL-6, NO, iNOS and COX-2). Also we examined the antioxidizing effect of the solvent extracts by determination of DPPH radical-scavenging activity. Among the solvent fractions, EtOAc and BuOH extracts showed potent radical scavenging activity (RC$_{50}$=10.9 and 12.7 respectively). The subtractions of EF 5-4-6-3-2 and BF 1 potentially inhibited the mRNA expression of pro-inflammatory cytokines (IL-1$\beta$, IL-6 and TNF-$\alpha$) at the concentration of 100 $\mu\textrm{g}$/mι. Also the fractions inhibited the mRNA expression of pro-inflammatory cytokines (IL-1$\beta$, IL-6 and TNF-$\alpha$) and protein expression of iNOS and COX-2 at the concentration of 100 $\mu\textrm{g}$/mι. And then, the inhibition of iNOS was correlated with the decrease of nitrite level. These results suggest that E. emarginata may have anti-inflammatory activity through the inhibition of pro-inflammatory cytokines, iNOS and COX-2.2.
Keywords
RAW264.7; Eurya emarginata; interferon-$\gamma$ (IFN-$\gamma$); Nitric Oxide (NO); Nitric Oxide Synthase (iNOS); cyclooxygenase (COX-2); tumor necrosis factor-$\alpha$; interleukin-6; interleukin-1$\beta$; inflammation;
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Times Cited By KSCI : 1  (Citation Analysis)
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