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In vitro Antiinflammatory Activity of the Essential Oil from Ligularia fischeri var. spiciformis in Murine Marcrophage Raw 264.7 Cells  

김륭규 (경희대학교 약학대학)
신경민 (경희대학교 약학대학)
천성국 (경희대학교 약학대학)
지사영 (경희대학교 약학대학)
서성훈 (경희대학교 약학대학)
박희준 (상지대학교 응용식물과학부)
최종원 (경성대학교 약학대학)
이경태 (경희대학교 약학대학)
Publication Information
YAKHAK HOEJI / v.46, no.5, 2002 , pp. 343-347 More about this Journal
Abstract
In the present study, effects of essential oils isolated from various plants have been evaluated on lipopolysaccharide (LPS)-induced release of nitric oxide (NO), prostaglandin E$_2$(PGE$_2$) and tumor necrosis factor-a (TNF-$\alpha$) by the macrophage RAW 264.7 cells. Among the tested essential oils, essential oil of Ligularia fischeri var. spiciformis (LF-oil) significantly inhibited the LPS-induced generation of NO, PGE$_2$ and TNF-$\alpha$ in Raw 264.7 cells. Consistent with these observations, the expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 enzyme was inhibited by LF-oil in a concentration-dependent manner. Thus, this study suggests that inhibition of release of iNOS, COX-2 expression, and TNF-$\alpha$ by the essential oil of Ligularia fischer may be one of the mechanisms responsible for the anti-inflammatory effects of this medicinal plant.
Keywords
Ligularia fischeri var. spiciformis; nitric oxide; RAW 264.7; COX-2; prostaglandin $E_2$; iNOS; TNF-$\alpha$;
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