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Flow Cytometrical Investigation on in vitro Immunomodulating Activity of PJ-4, a Protein-polysaccharide from Culture Flitrate of Insects-born Fungus Paecilomyces Tenuipes DGUM 32001  

정경수 (충남대학교 약학대학)
이지선 (충남대학교 약학대학)
김용해 (신화제약 중앙연구소)
한영환 (동국대학교 자연과학대학 생물학과)
이만형 (대구가톨릭대학교 약학대학)
Publication Information
YAKHAK HOEJI / v.46, no.3, 2002 , pp. 213-218 More about this Journal
Abstract
In the previous report, we described the in vivo antitumor activity of PJ-4, a protein-polysaccharide fraction prepared from the culture filtrate of an insect-born fungus, Paecilomyces tenuipes DGUM 32001. In the present study, we elucidated the immunomodulating activity of PJ-4 on the BALB/c mouse splenic lymphocytes using flow cytometrical techniques. As a result, PJ-4 was found to stimulate the lymphocytes not only to form lymphoblasts but also to express CD25 (IL-2 receptor $\alpha$ chain) molecule, which is well known as a T cell activation marker. More interestingly, its T cell stimulatory activity was more strongly exerted on CD8$^{+}$ T cells than on CD4$^{+}$ T cells. All these data suggest that PJ-4 exerts its antitumor activity at least partly through stimulation of T cells which play major roles in the cell-mediated immune system.tem.
Keywords
Basidiomycetes; Paecilomyces tenuipes; protein-polysaccharide; immunomodulation; flow cytometry;
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