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Antimetastatic Effect of Proteoglycan Isolated from the Mycelium of Ganoderma lucidum IY009 in vitro and in vivo  

백성진 (일양약품 중앙연구소)
김용석 (일양약품 중앙연구소)
용환미 (일양약품 중앙연구소)
채주병 (일양약품 중앙연구소)
이선애 (일양약품 중앙연구소)
배우철 (일양약품 중앙연구소)
박동우 (일양약품 중앙연구소)
김동연 (일양약품 중앙연구소)
이준우 (경북전문대학 식품가공과)
Publication Information
YAKHAK HOEJI / v.46, no.1, 2002 , pp. 11-17 More about this Journal
Abstract
$\beta$-Immunan, a proteoglycan, was isolated from the mycelium of Canoderma lucidum which belongs to a medicinal mushroom. The effects of $\beta$-Immunan on cell-cell and cell-matrix interactions mediated by carbohydrate-recognition and the mechanism responsible for the inhibition of experimental metastasis of Bl6-F10 and B16/BL6 murine melanoma were studied. The results showed that $\beta$-Immunan inhibited Bl6-Fl melanoma cell's adhesion to laminin and asialofetuin-induced homotypic aggregation and reduced invasion against Bl6-F10 murine melanoma cells through matrigel in vivo assay. When $\beta$-Immunan was intraperitoneally administrated to C57B/6 mice bearing B16/BL6 murine melanoma cells, it was decreased the number of pulmonary metastatic colony by the dose dependent manner ranging from 20 to 100 mg/kg/day. The results indirectly indicate that clinical treatment with $\beta$-Immunan might be expected to exhibit anti-metastatic effect. In the pulmonary metastasis, the number of pulmonary metastatic colony of melanoma when $\beta$-Immunan was intraperitoneally administrated to C57BL/6 mice bearing B16/BL6 murine melanoma cells by intravenous injection were decreased by the dose dependent manner ranging from 20 to 100 mg/kg/day.
Keywords
Ganoderma lucidum IY009; melanoma cell; materigel assay; proteoglycan; $\beta$-Immunan; antimetastatic effect;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 The role of interleukin-6 in inhibition of lung metastasis in subcutaneous tumor-bearing mice /
[ Inoue, k.;S. Okabe;E. Sueoka;N. Sueoka;T. Tabei;M. Suganuma ] / Oncol. Rep. C.
2 Induction of matrix metalloproteinase-9 requires a polymerized actin cytoskeleton in human malignant glioma cells /
[ Chintala S. K.;R. Sawaya, B. B.;Aggarwal, S. Majumder;D. K. Giri;A. P. Kyritsis;Z. L. Gokaslan;J. S. Rao ] / J. Biol. Chem.   DOI   ScienceOn
3 Adhesion molecules and tumor metastasis: and update /
[ Tang D. G.;K. V. Honn ] / Invasion Metastasis
4 Inhibition of melanoma pulmonray metastasis by methylxanthines due to decreased invasion and proliferation /
[ Lentini A.;H. K. Kleinman;P. Mattioli;V. Autuori-Pezzoli;L. Nicolini;A. Abbruzzese;M. Cardinali;S. Beninati ] / Melanoma Res.   DOI   ScienceOn
5 Endogenous galactoside-binding lectins a new calss of functional tumor cell surface molecules related to metastasis /
[ Raz A.;R. Lotan. ] / Cancer Metastasis Rev.   DOI   ScienceOn
6 Endogenous lectins as mediators of tumor cell adhesion /
[ Lotan R.;A. Raz. ] / J. Cell. Biochem.   DOI
7 Effect of combination therapy with matrix metalloproteinase inhibitor MMI-166 and mitomycin C on the growth and liver metastasis of human colon cancer /
[ Ohta M.;H. Konno;T. Tanaka;M. Baba;K. Kamiya;K. Oba;T. Kaneko;T. Syouji;A. Igarashi;S. Nakamura ] / Jpn. J. Cancer Res.   DOI
8 Effect of natural complex carbohydrate(citrus pectin) on murine melanoma cell properties related to galactin-3 functions /
[ Inohara, H.;A. Raz ] / Glycoconj. J   DOI   ScienceOn
9 Antimetastasis effect of lymphokine-activated killer(LAK) cells on fibrosarcoma in WKA rats /
[ Wang Z. H.;X. B. Li;X. D. Yao;D. N. Ba ] / Zhongua Zhong Liu Za Zhi
10 Blood concentration and toxicity of the antimetastasis agent NAMI-A following repeated intravenous treatment in mice /
[ Goccietto M.;G. Sava ] / Pharmacol. Toxicol   DOI   ScienceOn
11 The role of neutrophils as cytotoxic cells in lung metastasis: suppression of tumor cell metastasis by a biological response modifire(PSK) /
[ Ishihara Y.;T. Fujii;H. Iijima;K. Saito;K. Matsunaga ] / In vivo
12 The tetrazolium dye assay for rapid in vitro assessment of cytotoxicity /
[ Iselt, M.;W. Holtei;P. Hilgard ] / Arzneimittelforschung
13 Lack of in vitro cytotoxicity, associated to increased G(2)-M cell fraction and inhibition of matrigel invasion, may predic in vivo-selective antimetastasis activity of ruthenium complexes /
[ Zorzet S.;A. Bergamo;M. Cocchietto;A. Sorc;B. Gava;E. Alessio;E. Iengo;G. Sava ] / J. Pharmacol
14 Liver NK1.1+CD4+ alpha beta T cells activated by IL-12 as a major effector in inhibition of experimental tumor metastasis /
[ Takeda K.;S. Seki;K. Ogasawara;R. Anzai;W. Hashimoto;K. Sugiura;M. Takahashi;M. Satoh;K. Kumagai ] / J. Immunol
15 Ganoderma iuicidum IY009 배 양균사체 유래 단백다당류의 저분자와 고분자 분획의 특성 /
[ 백성진;김용석;전억한;이은숙;이준우 ] / 한국균학회지   과학기술학회마을
16 Influence of antiestrogens on the invasiveness and laminin attchment of breast cancer cells /
[ Rajah T. T.;J. T. Pento ] / Cancer Invest.   DOI   ScienceOn
17 Effects of tea polyphenols on the invasion and matrix metalloproteinases activities of human fibrosarcoma HT1080 cells /
[ Maeda-Yamamoto M;H. Kawahara;N. Tahara;K. Tsuji;Y. Hara;M. Isemura ] / J. Agric. Food Chem   DOI   ScienceOn
18 癌の 轉移抑制に關する實驗的硏究 /
[ 于原 吳郞 ] / 癌と化學療法
19 A basement-membrane permeability assay which correlates with the metastatic potential of tumour cells /
[ Parish C. R.;K. B. Jakobsen;D. R. Coombe. ] / Int. J. Cancer   DOI
20 The role of laminin in cancer invasion and metastasis /
[ Hunt G. ] / Exp. Cell Biol.
21 Studies on the mechanisms responsible for inhibition of experimental metastasis of B16-F10 murine melanoma by pentoxifyline /
[ Gude R.P.;M. M. Binda;H.L.Presas;A.J.P.Klein-Szanto;R. D. Bonfil ] / J. Biomed. Sci.   DOI   ScienceOn
22 Chemosensitivity testing of human colorimetric assay /
[ Park, J. G.;B. S. Kramer;S. M. Steinberg;J. Carmichael;J. M. Collins;J. D. Minna;A. F. Gazdar ] / Cancer Res.