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Simultaneous Quantification of Urinary L-, and D-Lactate by Reversed-Phase Liquid Chromatography Tandem Mass Spectrometry  

Moon, Chul Jin (Institutional affiliation: Green Cross Laboratories)
Yang, Song Hyun (Institutional affiliation: Green Cross Laboratories)
Publication Information
Journal of The Korean Society of Inherited Metabolic disease / v.15, no.2, 2015 , pp. 59-64 More about this Journal
Abstract
Purpose: Lactate has two optical isomers, L-lactate and D-lactate. In human L-lactate is the most abundant enantiomer of lactate. As plasma and urinary levels of L-lactate is associated with inherited metabolic disorders in general, D-lactate have been linked to the presence of diabetes and inflammatory bowel disease. Previously developed techniques have shown several limitations to further evaluate D-lactate as a biomarker for this condition. In this paper, we describe a highly sensitive, specific and fast liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the analysis of D-, L-lactate in urine. Methods: D- and L-lactate were quantified using high performance liquid chromatography tandem mass spectrometry (LC-MS/MS) with labelled internal standard. Samples were derivatized with (+)-O,O'-diacety-L-tartaric anhydride (DATAN) and seperated on a Poroshell 120 EC-C18 column. Results: Quantitative analysis of D-, and L-lactate was achieved successfully. Calibration curves were linear (r>0.999) over $0.5-100{\mu}g/mL$. Stabilities for samples were within the 10% varation. Inter- and Intra-day assay variations were below 10%. Conclusion: The presented method proved to be suitable for the quantitation of D- and L-lactate and opens the possibility to explore the use of D-lactate as a biomarker.
Keywords
D-lactate; L-Lacate; LC-MS/MS;
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1 Thornalley PJ. The glyoxalase system: new developments towards functional characterization of a metabolic pathway fundamental to biological life. Biochem J 1990;269:1-11.   DOI
2 Stallknecht B, Vissing J, Galbo H, Lactate production and clearance in exercise. Effects of training. A mini-review, Scand J Med Sci Sports 1998;8: 127-31.
3 Hasegawa H, Fukushima T, Lee JA, Tsukamoto K, Moriya K, Ono Y, et al. Determination of serum D-lactic and L-lactic acids in normal subjects and diabetic patients by column-switching HPLC with pre-column fluorescence derivatization, Anal Bioanal Chem 2003;377:886-91.   DOI
4 Kondoh Y, Kawase M, Kawakami Y, Ohmori S. Concentrations of D-lactate and its related metabolic intermediates in liver, blood, and muscle of diabetic and starved rats. Res Exp Med (Berl) 1992;192:407-14.   DOI
5 Murray MJ, Gonze MD, Nowak LR, Cobb CF. Serum D()-lactate levels as an aid to diagnosing acute intestinal ischemia. Am J Surg 1994;167:575- 8.   DOI
6 Duzgun AP, Bugdayci G, Sayin B, Ozmen MM, Ozer MV, Coskun F. Serum D-lactate: a useful diagnistic marker for acute appendicitis, Hepato- Gastroenterology 2007;54:1483-6.
7 Traube M, Bock JL, Boyer JL. D-Lactic acidosis after jejunoileal bypass: dentification of organic anions by nuclear magnetic resonance spectroscopy. Ann Intern Med 1983;98:171-3.   DOI
8 Ewaschuk JB, Naylor JM, Zello GA. D-Lactate in human and ruminant metabolism. J Nutr 2005; 135:1619-25.   DOI
9 Uribarri J, Oh MS, Carroll HJ. D-lactic acidosis. A review of clinical presentation, biochemical features, and pathophysiologic mechanisms. Medicine (Baltimore) 1998;77:73-82.   DOI
10 Inoue Y, Shinka T, Ohse M, Kohno M, Konuma K, Ikawa H, et al. Changes in urinary level and configuration ratio of d-lactic acid in patients with short bowel syndrome, J Chromatogr B 2007;855: 109-14.   DOI
11 Franco EJ, Hofstetter H, Hofstetter O. Determination of lactic acid enantiomers in human urine by highperformance immunoaffinity LC-MS, J Pharm Biomed Anal 2009;49:1088-91.   DOI