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http://dx.doi.org/10.3831/KPI.2018.21.028

The Preventive Effects of Standardized Extract of Zataria multiflora and Carvacrol on Acetaminophen-Induced Hepatotoxicity in Rat - Zataria multiflora and Carvacrol and Hepatotoxicity -  

Mohebbati, Reza (Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences)
Paseban, Maryam (Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences)
Beheshti, Farimah (Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences)
Soukhtanloo, Mohammad (Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences)
Shafei, Mohammad Naser (Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences)
Rakhshandeh, Hasan (Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences)
Rad, Abolfazl Khajavi (Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences)
Publication Information
Journal of Pharmacopuncture / v.21, no.4, 2018 , pp. 249-257 More about this Journal
Abstract
Objectives: The hepatotoxicity induced by Acetaminophen (AAP) mostly mediated by effect on oxidative stress parameters. The Zataria multiflora (Z.M) is an herbal medicine with well-known antioxidant effect. The aim of this study is investigation of preventive effects of Z.M and Carvacrol (CAR) on AAP-induced hepatotoxicity in rats. Methods: Rats were randomly divided into four groups including: 1) Control, 2) Acetaminophen (AAP), 3) and 4) CAR. The saline, Z.M (200 mg/kg) and CAR (20 mg/kg) were administrated orally for 6 days, after that AAP (600 mg/kg) was administrated in the $7^{th}$ day. Blood sampling was performed on the first and last days. Also, the liver tissue was removed for evaluation of Malondyaldehide (MDA), Thiol content, Superoxide dismutase (SOD) and Catalase (CAT). Total Protein (tPro), Glutamic Oxaloacetic Transaminase (GOT), Glutamic Pyruvic Transaminase (GPT) and Alkaline Phosphatase (ALP) in liver tissue were evaluated. The changes (${\Delta}$) of enzymes activities were presented. Results: The ${\Delta}GOT$, ${\Delta}GPT$ and ${\Delta}ALP$ in CAR group significantly decreased compared to AAP group (P < 0.01 to P < 0.001) and ${\Delta}GPT$ in Z.M group was significantly reduced in comparison with AAP group (P < 0.05). Also, MDA, Thiol, SOD and CAT levels in treated groups were attenuated compared to AAP group (P < 0.05 to P < 0.001). Conclusion: Z.M and CAR have a powerful hepatoprotective effect. CAR is more effective than Z.M. Based on the results. Z.M and CAR could be potent supplementary agents against hepatotoxicity of AAP in patients.
Keywords
acetaminophen; Zataria multiflora; carvacrol; hepatotoxicity;
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