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http://dx.doi.org/10.3831/KPI.2017.20.001

Boswellic Acid Improves Cognitive Function in a Rat Model Through Its Antioxidant Activity - Neuroprotective effect of Boswellic acid -  

Ebrahimpour, Saeedeh (Department of Pharmacology and Toxicology, Department of Basic Sciences, School of Veterinary Medicine, Shiraz University)
Fazeli, Mehdi (Department of Pharmacology and Toxicology, Department of Basic Sciences, School of Veterinary Medicine, Shiraz University)
Mehri, Soghra (Pharmaceutical Research Center, Mashhad University of Medical Sciences)
Taherianfard, Mahnaz (Department of Physiology, Department of Basic Sciences, School of Veterinary Medicine, Shiraz University)
Hosseinzadeh, Hossein (Pharmaceutical Research Center, Mashhad University of Medical Sciences)
Publication Information
Journal of Pharmacopuncture / v.20, no.1, 2017 , pp. 10-17 More about this Journal
Abstract
Objectives: Boswellic acid (BA), a compound isolated from the gum-resin of Boswellia carterii, is a pentacyclic terpenoid that is active against many inflammatory diseases, including cancer, arthritis, chronic colitis, ulcerative colitis, Crohn's disease, and memory impairment, but the mechanism is poorly understood. This study investigated the effects of boswellic acid on spatial learning and memory impairment induced by trimethyltin (TMT) in Wistar rats. Methods: Forty male Wistar rats were randomly divided into 5 groups: Normal group, TMT-administrated rats (8.0 mg/kg, Intraperitoneally, i.p.) and TMT + BA (40, 80 and 160 mg/kg, i.p.)-administrated rats. BA was used daily for 21 days. To evaluate the cognitive improving of BA, we performed the Morris water maze test. Moreover, to investigate the neuroprotective effect of BA, we determined the acetylcholinesterase (AchE) activity, the malondialdehyde (MDA) level as a marker of lipid peroxidation, and the glutathione (GSH) content in the cerebral cortex. Results: Treatment with TMT impaired learning and memory, and treatment with BA at a dose of 160 mg/kg produced a significant improvement in learning and memory abilities in the water maze tasks. Consistent with behavioral data, the activity of AChE was significantly increased in the TMT-injected rats compared to the control group (P < 0.01) whereas all groups treated with BA presented a more significant inhibitory effect against AChE than the TMT-injected animals. In addition, TMT reduced the GSH content and increased the MDA level in the cerebral cortex as compared to the control group) P < 0.01). On the other hand, treatment with BA at 160 mg/kg slightly increased the GSH content and reduced the MDA level in comparison to the TMT-administered group (P < 0.01). Conclusion: The above results suggest that the effect of BA in improving the cognitive function may be mediated through its antioxidant activity.
Keywords
acetylcholinesterase; antioxidant; boswellic acid; lipid peroxidation; trimethyltin;
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1 Bouldin TW, Goines ND, Bagnell RC, Krigman MR. Pathogenesis of trimethyltin neuronal toxicity. ultrastructural and cytochemical observations. Am J Pathol. 1981;104(3):237-49.
2 Dyer RS, Walsh TJ, Wonderlin WF, Bercegeay M. The trimethyltin syndrome in rats. Neurobehav Toxicol Teratol. 1982;4(2):127-33.
3 Fortemps E, Amand G, Bomboir A, Lauwerys R, Laterre EC. Trimethyltin poisoning. report of two cases. Int Arch Occup Environ Health. 1978;41(1):1-6.   DOI
4 Brown AW, Aldridge WN, Street BW, Verschoyle RD. The behavioral and neuropathologic sequelae of intoxication by trimethyltin compounds in the rat. Am J Pathol. 1979;97(1):59-82.
5 Shin EJ, Suh SK, Lim YK, Jhoo WK, Hjelle OP, Ottersen OP, et al. Ascorbate attenuates trimethyltin-induced oxidative burden and neuronal degeneration in the rat hippocampus by maintaining glutathione homeostasis. Neuroscience. 2005;133(3):715-27.   DOI
6 Yoneyama M, Nishiyama N, Shuto M, Sugiyama C, Kawada K, Seko K, et al. In vivo depletion of endogenous glutathione facilitates trimethyltin-induced neuronal damage in the dentate gyrus of mice by enhancing oxidative stress. Neurochem Int. 2008;52(4-5):761-9.   DOI
7 Kaur S, Chhabra R, Nehru B. Ginkgo biloba extract attenuates hippocampal neuronal loss and cognitive dysfunction resulting from trimethyltin in mice. Phytomedicine. 2013;20(2):178-86.   DOI
8 Hosseinzadeh H, Karimi G, Nobakht N. Effects of Rosmarinus officinalis L. aerial parts essential oil on intact memory and scopolamine-induced learning deficits in rats performing the morris water maze task. J Medicinal Plants. 2004;4(12):51-7.
9 Stavric B. Role of chemopreventers in human diet. Clin Biochem. 1994;27(5):319-32.   DOI
10 Hosseinzadeh H, Sadeghnia HR, Ghaeni FA, Motamedshariaty VS, Mohajeri SA. Effects of saffron (Crocus sativus L.) and its active constituent, crocin, on recognition and spatial memory after chronic cerebral hypoperfusion in rats. Phytother Res. 2012;26(3):381-6.   DOI
11 Zhao W, Entschladen F, Liu H, Niggemann B, Fang Q, Zaenker KS, et al. Boswellic acid acetate induces differentiation and apoptosis in highly metastatic melanoma and fibrosarcoma cells. Cancer Detect Prev. 2003;27(1):67-75.   DOI
12 Poeckel D, Werz O. Boswellic acids: biological actions and molecular targets. Curr Med Chem. 2006;13(28):3359-69.   DOI
13 Hartmann RM, Morgan Martins MI, Tieppo J, Fillmann HS, Marroni NP. Effect of Boswellia serrata on antioxidant status in an experimental model of colitis rats induced by acetic acid. Dig Dis Sci. 2012;57(8):2038-44.   DOI
14 Forouzanfar F, Hosseinzadeh H, Ebrahimzadeh Bideskan A, Sadeghnia HR. Aqueous and ethanolic extracts of Boswellia serrata protect against focal cerebral ischemia and reperfusion injury in rats. Phytother Res. 2016;30(12):1954-67.   DOI
15 Hosseinzadeh H, Ramezani M, Akhtar Y, Ziaei T. Effects Boswellia carterii gum resin fractions on intact memory and hyoscine-induced learning impairments in rats performing the morris water maze task. J Med Plants. 2010;2(34):95-101.
16 Assimopoulou AN, Zlatanos SN, Papageorgiou VP. Antioxidant activity of natural resins and bioactive triterpenes in oil substrates. Food Chem. 2005;92(4):721-7.   DOI
17 Morris R. Developments of a water-maze procedure for studying spatial learning in the rat. J Neurosci Methods. 1984;11(1):47-60.   DOI
18 Jaberian H, Piri K, Nazari J. Phytochemical composition and in vitro antimicrobial and antioxidant activities of some medicinal plants. Food Chem. 2013;136(1):237-44.   DOI
19 Sabina EP, Indu H, Rasool M. Efficacy of boswellic acid on lysosomal acid hydrolases, lipid peroxidation and anti-oxidant status in gouty arthritic mice. Asian Pac J Trop Biomed. 2012;2(2):128-33.   DOI
20 Shim HS, Park HJ, Ahn YH, Her S, Han JJ, Hahm DH, et al. Krill-derived phosphatidylserine improves TMT-induced memory impairment in the rat. Biomol Ther. 2012;20(2):207-13.   DOI
21 Jenkins SM, Barone S. The neurotoxicant trimethyltin induces apoptosis via caspase activation, p38 protein kinase, and oxidative stress in PC12 cells. Toxicol lett. 2004;147(1):63-72.   DOI
22 Mihara M, Uchiyama M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem. 1978;86(1):271-8.   DOI
23 Razavi M, Hosseinzadeh H, Abnous K, Motamedshariaty VS, Imenshahidi M. Crocin restores hypotensive effect of subchronic administration of diazinon in rats. Iran J Basic Med Sci. 2013;16(1):64-72.
24 Gasso S, Sanfeliu C, Sunol C, Rodriguez-Farre E, Cristofol RM. Trimethyltin and triethyltin differentially induce spontaneous noradrenaline release from rat hippocampal slices. Toxicol Appl Pharmacol. 2000;162(3):189-96.   DOI
25 Thompson TA, Lewis JM, Dejneka NS, Severs WB, Polavarapu R, Billingsley ML. Induction of apoptosis by organotin compounds in vitro: neuronal protection with antisense oligonucleotides directed against stannin. J Pharmacol Exp Ther. 1996;276(3):1201-16.
26 Qu M, Zhou Z, Chen C, Li M, Pei L, Chu F, et al. Lycopene protects against trimethyltin-induced neurotoxicity in primary cultured rat hippocampal neurons by inhibiting the mitochondrial apoptotic pathway. Neurochem Int. 2011;59(8):1095-103.   DOI
27 Kim JK, Choi SJ, Bae H, Kim CR, Cho HY, Kim YJ, et al. Effects of methoxsalen from Poncirus trifoliata on acetylcholinesterase and trimethyltin-induced learning and memory impairment. Biosci Biotechnol Biochem. 2011;75(10):1984-9.   DOI
28 Karpiak VC, Eyer CL. Differential gliotoxicity of organotins. Cell Biol Toxicol. 1999;15(4):261-8.   DOI
29 Cookson MR, Slamon ND, Pentreath VW. Glutathione modifies the toxicity of triethyltin and trimethyltin in C6 glioma cells. Arch Toxicol. 1998;72(4):197-202.   DOI
30 Gunasekar PG, Mickova V, Kotyzova D, Li L, Borowitz JL, Eybl V, et al. Role of astrocytes in trimethyltin neurotoxicity. J Biochem Mol Toxicol. 2001;15(5):256-62.   DOI
31 Choi GN, Kim JH, Kwak JH, Jeong CH, Jeong HR, Lee U, et al. Effect of quercetin on learning and memory performance in ICR mice under neurotoxic trimethyltin exposure. Food Chem. 2012;132(2):1019-24.   DOI
32 Bohnen NI, Kaufer DI, Hendrickson R, Ivanco LS, Lopresti B, Davis JG, et al. Cognitive correlates of alterations in acetylcholinesterase in Alzheimer's disease. Neurosci Lett. 2005;380(1-2):127-32.   DOI
33 Morita Y, Yanagida D, Shintani N, Ogita K, Nishiyama N, Tsuchida R, et al. Lack of trimethyltin (TMT)-induced elevation of plasma corticosterone in PACAP-deficient mice. Ann N Y Acad Sci. 2006;1070(1):450-6.   DOI
34 Alessandri B, FitzGerald RE, Schaeppi U, Krinke GJ, Classen W. The use of an unbaited tunnel maze in neurotoxicology: I. trimethyltin-induced brain lesions. Neurotoxicology. 1993;15(2):349-57.
35 Hagan JJ, Jansen JH, Broekkamp CL. Selective behavioural impairment after acute intoxication with trimethyltin (TMT) in rats. Neurotoxicology. 1987;9(1):53-74.
36 Swartzwelder H. Impaired maze performance in the rat caused by trimethyltin treatment: problem-solving deficits and perseveration. Neurobehavioral Toxicology & Teratology. 1982;4(2):169-76.
37 Balaban CD, O'Callaghan JP, Billingsle ML. Trimethyltin-induced neuronal damage in the rat brain: comparative studies using silver degeneration stains, immunocytochemistry and immunoassay for neuronotypic and gliotypic proteins. Neuroscience. 1988;26(1):337-61.   DOI
38 Chang LW, Dyer RS. Trimethyltin induced pathology in sensory neurons. Neurobeh Toxicol Teratol. 1983;5(6):673-96.
39 Koczyk D. How does trimethyltin affect the brain: facts and hypotheses. Acta Neurobiol Exp. 1995;56(2):587-96.
40 Earley B, Burke M, Leonard BE. Behavioural, biochemical and histological effects of trimethyltin (TMT) induced brain damage in the rat. Neurochem Int. 1992;21(3):351-66.   DOI
41 Walsh TJ, Miller DB, Dyer RS. Trimethyltin, a selective limbic system neurotoxicant, impairs radial-arm maze performance. Neurobeh Toxicol Teratol. 1982;4(2):177-83.
42 Woodruff ML, Baisden RH, Cannon RL, Kalbfleisch J, Freeman JN 3rd. Effects of trimethyltin on acquisition and reversal of a light-dark discrimination by rats. Physiol Behav. 1994;55(6):1055-61.   DOI
43 Ammon H. Boswellic acids in chronic inflammatory diseases. Planta Med. 2006;72(12):1100-16.   DOI