Browse > Article
http://dx.doi.org/10.3831/KPI.2016.19.025

Agarwood Inhibits Histamine Release from Rat Mast Cells and Reduces Scratching Behavior in Mice -Effect of Agarwood on Histamine Release and Scratching Behavior-  

Inoue, Eiji (Tokyo Research Center, Kyushin Pharmaceutical Co, Ltd.)
Shimizu, Yasuharu (Tokyo Research Center, Kyushin Pharmaceutical Co, Ltd.)
Masui, Ryo (Tokyo Research Center, Kyushin Pharmaceutical Co, Ltd.)
Tsubonoya, Tomoe (Tokyo Research Center, Kyushin Pharmaceutical Co, Ltd.)
Hayakawa, Tomomi (Tokyo Research Center, Kyushin Pharmaceutical Co, Ltd.)
Sudoh, Keiichi (Tokyo Research Center, Kyushin Pharmaceutical Co, Ltd.)
Publication Information
Journal of Pharmacopuncture / v.19, no.3, 2016 , pp. 239-245 More about this Journal
Abstract
Objectives: This study was conducted to clarify the effects of agarwood on histamine release from mast cells in rats and on the scratching behaviors in mice. Methods: Histamine release from rat mast cells induced by compound 48/80 or concanavalin A (Con A) and compound 48/80-induced scratching behavior in mice were examined to investigate the effects of agarwood. The hyaluronidase activity and the 3',5'-cyclic adenosine monophosphate (cAMP) levels in mast cells were examined to investigate the mechanisms for the inhibition of histamine release. The correlation between the inhibitory effects of agarwood on histamine release and the content of its typical ingredients, a 2-(2-phenylethyl)chromone derivatives, was analyzed using thin-layer chromatography. Results: Agarwood showed an inhibitory effect on mast-cell histamine release induced by compound 48/80 or Con A without any effect on hyaluronidase activity; this effect involves an increase in the cAMP levels in mast cells. Oral administration of agarwood showed an inhibitory effect on compound 48/80-induced scratching behavior in mice. The inhibitory effects of agarwood on histamine release were quite different, depending on the area where the agarwood was produced, its quality, and its market price. No correlation was found between the inhibitory effects of agarwood on histamine release and the typical ingredients of agarwood, which are 2-(2-phenylethyl)chromone derivatives. Conclusion: These results show that agarwood inhibits histamine release from mast cells partially through an increase in the cAMP levels in cells. We suggest that some active ingredients of agarwood must be effective on oral intake and that agarwood can be used to treat patients with a number of conditions, including urticaria, atopic dermatitis, and bronchial asthma, in which an increase in histamine release occurs. Differences in the pharmacological effects of this crude drug among markets may provide important information for the quality control of this herbal medicine.
Keywords
agarwood; histamine release; mast cells; scratching behavior;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Luskin AT, Luskin SS. Anaphylaxis and anaphylactoid reactions: diagnosis and management. Am J Ther. 1996;3(7):515-20.   DOI
2 White MV. The role of histamine in allergic diseases. J Allergy Clin Immunol. 1990;86(4):599-605.   DOI
3 Noguchi T, Shimizu Y, Sudoh K. [Basic pharmacological studies of Agarwood for its medicinal application for asthma]. The 129th Annual Meeting of the Pharmaceutical Society of Japan. 2009;abstracts papers 2:164. Japanese.
4 Yoneda K, Yamagata E, Sugimoto Y, Nakanishi T. [Pharmacognostical studies on the crude drug of "agarwood" (I): comparison of the constituents of the essential oil from agarwood by means of GLC and GC-MS]. Shoyakugaku Zasshi. 1986;40(3):252-8. Japanese.
5 Saito SY, Tanaka M, Matsunaga K, Li Y, Ohizumi Y. The combination of rat mast cell and rabbit aortic smooth muscle is the simple bioassay for the screening of anti-allergic ingredient from methanolic extract of corydalis tuber. Biol Pharm Bull. 2004;27(8):1270-4.   DOI
6 Shore PA, Burkhalter A, Cohn VH Jr. A method for the fluorometric assay of histamine in tissues. J Pharmacol Exp Ther. 1959;127:182-6.
7 The Japanese Pharmacopoeia sixteenth Edition. 2011. Japanese.
8 Kubo Y, Shozen K, Seto Y. [Hyaluronidase inhibitory effect in diatom extracts isolated from deep sea water]. Deep Ocean Water Research. 2002;3(2):71-6. Japanese.
9 Maeda Y, Yamamoto M, Masui T, Sugiyama K, Yokota M, Nakagomi K, et al. [Inhibitory effect of tea extracts on hyaluronidase. (studies on anti-allergic activity in tea. 2)]. J Food Hyg Soc Jpn. 1990;31(3):233-7. Japanese.   DOI
10 Dai Y, Hou LF, Chan YP, Cheng L, But PP. Inhibition of immediate allergic reactions by ethanol extract from Plumbago zeylanica stems. Biol Pharm Bull. 2004;27(3):429-32.   DOI
11 Kubo M, Matsuda H, Dai Y, Ido Y, Yoshikawa M. [Studies on kochiae fructus. I. antipruritogenic effect of 70% ethanol extract from kochiae fructus and its active component]. Yakugaku Zasshi. 1997;117(4):193-201. Japanese.   DOI
12 Jeon IH, Kim HS, Kang HJ, Lee HS, Jeong SI, Kim SJ, et al. Anti-inflammatory and antipruritic effects of luteolin from Perilla (P. frutescens L.) leaves. Molecules. 2014;19(6):6941-51.   DOI
13 Kim YC, Lee EH, Lee YM, Kim HK, Song BK, Lee EJ, et al. Effect of the aqueous extract of Aquilaria agallocha stems on the immediate hypersensitivity reactions. J Ethnopharmacol. 1997;58(1):31-8.   DOI
14 Kakegawa H, Matsumoto H, Satoh T. Activation of hyaluronidase by metallic salts and compound 48/80, and inhibitory effect of anti-allergic agents on hyaluronidase. Chem Pharm Bull. 1985;33(2):642-6.   DOI
15 Allansmith MR, Baird RS, Ross RS, Barney NP, Bloch KJ. Ocula anaphylaxis induced in the rat by topical applacation of compound 48/80. Acta Ophthalmol Suppl. 1989;192:145-53.
16 Windelborg Nielsen B, Hansen B, Damsgaard TM, Herlin T, Soderberg U, Bjerke T, et al. Basophil histamine release induced by anti-IgE and concanavalin A. relation to the total plasma IgE content. Allergy. 1993;48(1):54-61.   DOI
17 Weston MC, Peachell PT. Regulation of human mast cell and basophil function by cAMP. Gen Pharmacol. 1998;31(5):715-9.   DOI