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http://dx.doi.org/10.3831/KPI.2016.19.013

Cornu cervi pantotrichum Pharmacopuncture Solution Facilitate Hair Growth in C57BL/6 Mice  

Lee, Seon-Yong (Apple Korean Medical Clinic)
Lee, Dong-Jin (Department of Ophthalmology, Otolaryngology and Dermatology, Korean Medicine Hospital, Pusan National University)
Kwon, Kang (Department of Ophthalmology, Otolaryngology and Dermatology, Korean Medicine Hospital, Pusan National University)
Lee, Chang-Hyun (Department of Anatomy, College of Korean Medicine, Woosuk University)
Shin, Hyun Jong (Department of Pathology, College of Korean Medicine, Woosuk Univeristy)
Kim, Jai Eun (Department of Pathology, College of Korean Medicine, Dongguk University)
Ha, Ki-Tae (Division of Applied Medicine, School of Korean Medicine, Pusan National University)
Jeong, Han-Sol (Division of Applied Medicine, School of Korean Medicine, Pusan National University)
Seo, Hyung-Sik (Department of Ophthalmology, Otolaryngology and Dermatology, Korean Medicine Hospital, Pusan National University)
Publication Information
Journal of Pharmacopuncture / v.19, no.2, 2016 , pp. 122-128 More about this Journal
Abstract
Objectives: Cornu cervi pantotrichum (CCP) has been widely used in Korean and China, as an anti-fatigue, anti-aging, and tonic agent to enhance the functions of the reproductive and the immune systems. Because CCP has various growth factors that play important roles in the development of hair follicles, we examined whether CCP pharmacopuncture solution (CCPPS) was capable of promoting hair growth in an animal model. Methods: One day after hair depilation, CCPPS were topically applied to the dorsal skin of C57BL/6 mice once a day for 15 days. Hair growth activity was evaluated by using macro- and microscopic observations. Dorsal skin tissues were stained with hematoxylin and eosin. Expressions of bromodeoxyuridine (BrdU), proliferating cell nuclear antigen (PCNA), and fibroblast growth factor (FGF)-7 were examined by using immunohistochemical staining. A reverse transcription polymerase chain reaction (RT-PCR) analysis was also conducted to measure the messenger RNA (mRNA) expression of FGF-7. Results: CCPPS induced more active hair growth than normal saline. Histologic analysis showed enlargement of the dermal papilla, elongation of the hair shaft, and expansion of hair thickness in CCPPS treated mice, indicating that CCPPS effectively induced the development of anagen. CCPPS treatment markedly increased the expressions of BrdU and PCNA in the hair follicles of C57BL/6 mice. In addition, CCPPS up regulated the expression of FGF-7, which plays an important role in the development of hair follicles. Conclusion: These results reveal that CCPPS facilitates hair re-growth by proliferation of hair follicular cells and up-regulation of FGF-7 and suggest that CCPPS can potentially be applied as an alternative treatment for patients with alopecia.
Keywords
anagen; Cornu cervi pantotrichum pharmacopuncture solution; fibroblast growth factor-7; hair; hair loss; proliferating cell nuclear antigen;
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1 Kazantseva A, Goltsov A, Zinchenko R, Grigorenko AP, Abrukova AV, Moliaka YK, et al. Human hair growth deficiency is linked to a genetic defect in the phospholipase gene LIPH. Science. 2006;314(5801):982-5.   DOI
2 Kaufman KD. Androgens and alopecia. Mol Cell Endocrinol. 2002;198(1-2):89-95.   DOI
3 Hadshiew IM, Foitzik K, Arck PC, Paus R. Burden of hair loss: stress and the underestimated psychosocial impact of telogen effluvium and androgenetic alopecia. J Invest Dermatol. 2004;123(3):455-7.   DOI
4 Peters EM, Arck PC, Paus R. Hair growth inhibition by psychoemotional stress: a mouse model for neural mechanisms in hair growth control. Exp Dermatol. 2006;15(1):1-13.   DOI
5 Wang L, Million M, Rivier J, Rivier C, Craft N, Mary P, et al. CRF receptor antagonist astressin-B reverses and prevents alopecia in CRF over-expressing mice. PLoS One. 2011;16(2):DOI:10.1371/journal.pone.0016377.   DOI
6 Alexis A, Dudda-Subramanya R, Sinha A. Alopecia areata: autoimmune basis of hair loss. Eur J Dermatol. 2004;14(6):364-70.
7 Gilhar A, Kalish RS. Alopecia areata: a tissue specific autoimmune disease of the hair follicle. Autoimmun Rev. 2006;5(1):64-9.   DOI
8 Messenger A, Rundegren J. Minoxidil: mechanisms of action on hair growth. Brit J Dermatol. 2004;150(2):186-94.   DOI
9 Martino M, Briquez P, Guc E, Tortelli F, Kilarski W, Metzger S, et al. Growth factors engineered for super-affinity to the extracellular matrix enhance tissue healing. Science. 2014;343(6173):885-8.   DOI
10 Von den driesch P, Fartasch M, Diepgen TL, Peter KP. Protein contact dermatitis from calf placenta extracts. Contact Dermatitis. 1993;28(1):46-7.   DOI
11 Suh SJ, Kim KS, Lee AR, Ha KT, Kim JK, Kim DS, et al. Prevention of collagen-induced arthritis in mice by Cervus Korean TEMMINCK var. mantchuricus Swinhoe. Environ Toxicol Pharmacol. 2007;23(2):147-53.   DOI
12 Kim KH, Kim KS, Choi BJ, Chung KH, Chang YC, Lee SD, et al. Anti-bone resorption activity of deer antler aqua-acupunture, the pilose antler of Cervus Korean TEMMINCK var. mantchuricus Swinhoe (Nokyong) in adjuvant-induced arthritic rats. J Ethnopharmacol. 2005;96(3):497-506.   DOI
13 Li C, Stanton JA, Robertson TM, Suttie JM, Sheard PW, Harris AJ, et al. Nerve growth factor mRNA expression in the regenerating antler tip of red deer (Cervus elaphus). PLoS One. 2007;2(1):e148.   DOI
14 Wu F, Li H, Jin L, Li X, Ma Y, You J, et al. Deer antler base as a traditional Chinese medicine: a review of its traditional uses, chemistry and pharmacology. J Ethnopharmacol. 2013;145(2):403-15.   DOI
15 Paus R, Arck P. Neuroendocrine perspectives in alopecia areata: does stress play a role?. J Invest Dermatol. 2009;129(6):1324-6.   DOI
16 Ahmad W, Faiyaz HM, Brancolini V, Tsou HC, Haque SU, Lam H, et al. Alopecia universalis associated with a mutation in the human hairless gene. Science. 1998;279(5351):720-4.   DOI
17 Ellis JA, Stebbing M, Harrap SB. Polymorphism of the androgen receptor gene is associated with male pattern baldness. J Invest Dermatol. 2001;116(3):452-5.   DOI
18 Willemsen R, Vanderlinden J, Roseeuw D, Haentjens P. Increased history of childhood and lifetime traumatic events among adults with alopecia areata. J Am Acad Dermatol. 2009;60(3):388-93.   DOI
19 Han SH, Byun JW, Lee WS, Kang H, Kye YC, Kim KH, et al. Quality of life assessment in male patients with androgenetic alopecia: result of a prospectve, multicenter study. Ann Dermatol. 2012;24(3):311-8.   DOI
20 Price VH. Treatment of hair loss. N Engl J Med. 1999;341(13):964-73.   DOI
21 Wilson C, Walkden V, Powell S, Shaw S, Wilkinson J, Dawber R. Contact dermatitis in reaction to 2% topical minoxidil solution. J Am Acad Dermatol. 1991;24(4):661-2.   DOI
22 Kaufman KD, Olsen EA, Whiting D, Savin R, DeVillez R, Bergfeld W, et al. Finasteride in the treatment of men with androgenetic alopecia. J Am Acad Dermatol. 1998;39(4):578-89.   DOI
23 Georgala S, Befon A, Maniatopoulou E, Georgala C. Topical use of minoxidil in children and systemic side effects. Dermatology. 2007;214(1):101-2.   DOI
24 Irwig MS. Depressive symptoms and suicidal thoughts among former users of finasteride with persistent sexual side effects. J Clin Psychiatry. 2012;73(9):1220-3.   DOI
25 Gu L, Mo E, Yang Z, Zhu X, Fang Z, Sun B, et al. Expression and localization of insulin-like growth factor-I in four parts of the red deer antler. Growth Factors. 2007;25(4):264-79.   DOI
26 Irwig MS. Persistent sexual side effects of finasteride: could they be permanent?. J Sex Med. 2012;9(11):2927-32.   DOI
27 Kim JH, Jung TY, Seo J, Lee S, Kim MG, Leem KH, et al. Involvement of MAPK signaling pathway in the osteogenic gene expressions of Cervi Pantotrichum Cornu in MG-63 human osteoblast-like cells. Life Sci. 2013;94(1):45-53.   DOI
28 Jhon GJ, Park SY, Han SY, Lee S, Kim Y, Chang YS. Studies of the chemical structure of gangliosides in deer antler, Cervus nippon. Chem Pharm Bull (Tokyo). 1999;47(1):123-7.   DOI
29 Pita-Thomas W, Fernandez-Martos C, Yunta M, Maza RM, Navarro-Ruiz R, Lopez-Rodriguez MJ, et al. Gene expression of axon growth promoting factors in the deer antler. PLoS One. 2010;5(12):DOI:10.1371/journal.pone.0015706.   DOI
30 Cox HD, Eichner D. Detection of human insulin-like growth factor-1 in deer antler velvet supplements. Rapid Commun Mass Spectrom. 2013;27(19):2170-8.   DOI
31 Weger N, Schlake T. IGF-I signalling controls the hair growth cycle and the differentiation of hair shafts. J Invest Dermatol. 2005;125(5):873-82.   DOI
32 Nowakowski RS, Lewin SB, Miller MW. Bromodeoxy-uridine immunohistochemical determination of the lengths of the cell cycle and the DNA-synthetic phase for an anatomically defined population. J Neurocytol. 1989;18(3):311-8.   DOI
33 Bravo R, Frank R, Blundell PA, Macdonald-Bravo H. Cyclin/Pcna Is the auxiliary protein of DNA polymerase-delta. Nature. 1987;326(6112):515-7.   DOI
34 Booth C, Potten CS. Keratinocyte growth factor increases hair follicle survival following cytotoxic insult. J Invest Dermatol. 2000;114(4):667-73.   DOI