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http://dx.doi.org/10.3831/KPI.2010.13.1.063

Component analysis of cultivated ginseng, cultivated wild ginseng, and wild ginseng and the change of ginsenoside components in the process of red ginseng  

Jeong, H.S. (College of Oriental medicine, Sangji Univ.)
Lim, C.S. (College of Oriental medicine, Sangji Univ.)
Cha, B.C. (Pharmaceutical Engineering, Health of Science, Sangji Univ.)
Choi, S.H. (Division of Animal resources and life science, Sangji Univ.)
Kwon, K.R. (College of Oriental medicine, Sangji Univ.)
Publication Information
Journal of Pharmacopuncture / v.13, no.1, 2010 , pp. 63-77 More about this Journal
Abstract
Objectives: The aim of this experiment is to provide an objective differentiation of cultivated ginseng, cultivated wild ginseng, and wild ginseng through component analysis, and to know the change of ginsenoside components in the process for making red ginseng. Methods: Comparative analysis of ginsenoside $Rb_1,\;Rb_2$, Rc, Rd, Re, Rf, $Rg_1,\;Rg_3,\;Rh_1$ and $Rh_2$ from the cultivated ginseng 4 and 6 years, cultivated wild ginseng, and wild ginseng were conducted using High Performance Liquid Chromatography(hereafter HPLC). And the same analyses were conducted in the process of red ginseng. Results: 1. For content comparison of ginsenoside $Rb_1$, Rc, Rd, Rf, $Rg_1$ and $Rh_1$, wild ginseng showed high content, followed cultivated ginseng 4 and 6 years, cultivated wild ginseng showed low content than any other samples. 2. For content comparison of ginsenoside $Rb_2$ and Re, cultivated ginseng 4 years showed high content, followed wild ginseng and cultivated ginseng 6 years, cultivated wild ginseng showed low content than any other samples. 3. For content comparison of ginsenoside $Rg_3$, wild ginseng and cultivated wild ginseng were only showed low content. 4. For content comparison of ginsenoside $Rh_2$, cultivated wild ginseng was only showed low content. 5. In the process of red ginseng, ginsenoside $Rb_1,\;Rb_2$, Rc, Rd, $Rg_3$ and $Rh_1$ were increased, and ginsenoside Re and $Rg_1$ were decreased in cultivated wild ginseng. 6. In the process of red ginseng, ginsenoside $Rg_3$ and $Rh_1$ were increased, and ginsenoside $Rb_2$, Rc, and Re were decreased in cultivated ginseng 4 years. 7. In the process of red ginseng, ginsenoside $Rb_1,\;Rb_2$, Rf and $Rh_1$ were increased, and ginsenoside Rc and Rd were decreased in cultivated ginseng 6 years. Conclusions: Distribution of ginsenoside contents to the cultivated ginseng, cultivated wild ginseng, and wild ginseng was similar and was not showed special characteristics between samples. And the change of ginsenoside to the process of red ginseng, cultivated ginseng and cultivated wild ginseng were showed different aspect.
Keywords
ginsenoside; cultivated wild ginseng; wild ginseng; cultivated ginseng; HPLC;
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1 School of Korean Medicine. Dept. of Herbology. Herbology. Youngrim. 1994; 531
2 Brekhman. II. Panax ginseng, Gosudarst Isdat et Med, Lit. Leningard, 1957; 1-5
3 고려인삼학회, 고려삼의 이해, Advances in Ginseng Research, 1998; 127-137
4 최진호. 인삼의 신비, 서울, 교문사, 1984; 13-14
5 Nam KY. 최신 고려인삼. 천일인쇄소. 1996; 56
6 Ha TY, Lee JH, and Han JH. Inhibitory effects of Panax ginseng on tumorigenesis in mice. J Cheonbuk medical Sci. 1987; 11(1): 1-11
7 Shin SS, Kim KC, Choi YH, Lee YT, Eom HS and Kim CS. Critic standardization and object ivity of mountain grown ginseng. KIOM vol.5. 2001; 107-114
8 Kim, JS., Kim, KW., Choi, KJ., Kwak, YK., Im, KS., Lee, KM. and Chung, HY. Screening of anti-oxidative components from red ginseng saponin. Korean J. Ginseng Sci. 1996; 20: 173-178   과학기술학회마을
9 Kim, MJ. and Jung, NP. The effects of ginseng saponin on the mouse immune system. Korean J. Ginseng Sci. 1987; 11: 130-135
10 Jeong CS, Hyun JE and Kim YS. Anti-oxidative effect of ginsenoside $Rb_1$ on the H치, ethanol-induced gastric tissue in rats. Kor. J Pharmacogn. 2002; 33(3): 252-256   과학기술학회마을
11 David GP and David DK. Ginsenosides 20(S)-protopanaxadiol and Rh2 reduce cell proliferation and increase sub-G1 cells in two cultured intestinal cell lines, Int-407 and Caco-2. Can. J Physiol. Pharmacol. 2004; 82: 183-190   DOI   ScienceOn
12 Choi KJ. The constituent of material ginseng and management of quality. Korean J Ginseng Sci. 1991; 15(3): 247-256
13 Hideo H, Seong JH, Yasatosi M, Masamori W and Hur JD. Metabolites of ginseng saponin by enterobacteria and anti cancer substance include it's useful constituent. Korea Plant. 1998; No 10-164266-0000
14 Oliveira, ACC., Perez, AC., Merino G., Prietp, JG. and Alvarez, AI. Protective effects of Panax ginseng on muscle injury and inflammation after eccentric exercise. Comparative Biochemistry and Physiology Part C. 2001; 130: 369-377
15 Morita, T. Chemical studies on Panax genus plants grown in Asia. Hiroshima univ. Doctoral Thesis, 6-7(1986)
16 Attele AS, Wu JA and Yuan CS. Ginseng pharmacology: multiple constituents and mutiple actions. Biochem. Pharmacol. 1999; 58: 1685-1693   DOI   ScienceOn
17 이상인. 본초학. 수서원. 1985; 60
18 Shibata, S., Tanaka, O., Soma, K., lita, Y., And o, T. and Nakamura, H. Studies on saponins and sapogenins of ginseng. The structure of panaxatriol. Tetrahedron Lett. 1965; 3: 207-213
19 Fuzzati, N., Gabetta, B., Jayakar, K., Pace, R. and Peterlongo, F. Liquid chromatographyelectrospray mass spectrometric identification of ginsenosides in Panax ginseng roots. J. Chromatogr. 1999; 854: 69-79   DOI   ScienceOn
20 Wang, H., Zou, H., Kong, L., Zhang, Y., Pang, H., Su, C., Liu, G., Hui, M. and Fu, Li. Determination of ginsenoside Rg3 in plasma by solid-phase extraction and high-performance liquid chromatography for pharmaco-kinetic study. J. Chromatogr. 1999; 731: 403-409   DOI   ScienceOn
21 Park JD. Rescent studies on the chemical constituents of Korean Ginseng. Korean J. Ginseng Sci. 1996: 20(4): 389-415
22 LY Wu J. Inhibitory effect of 20(S)-ginsenoside $Rg_3$ on growth and metasis of Lewis pulmonary carcinoma. Zhonggliu Fangzhi Yanjiu. 2006; 33: 311-313
23 Choi SS, Lee JK, Han KJ, Lee HK, Lee J and Suh HW. Effects of ginsenoside Rd on nitric oxide system induced by lipopolysaccharide plus TNF-${/alpha}$ in C6 rat glioma cells. Arch Pharm. Res. 2003; 26: 375-382   DOI   ScienceOn
24 Yokozawa T and Liu ZW. The role of ginsenoside Rd in cisplatin-induces acute renal failure. Ren. Fail. 2000; 22: 115-127   DOI   ScienceOn
25 Wang W, Zhao Y, Rayburn ER, Hill DL, Wang H and Zhang R. In vitro anti-cancer activity and structure-activity relationships of natural products isolated from fruits of Panax ginseng. Cancer Chemoth Pharmacol. 2007; 59: 589-601   DOI
26 Ko, S. R., Choi, K. J., Kim, S. C. and Han, K. H. Content and composition of saponin compounds of Panax species. Korean J. Ginseng Sci. 1995; 19: 254-295   과학기술학회마을
27 Kwon, S. W., Han, S. B., Park, I. H., Kim, J. M., Park, M. K. and Park, J. l. Liquid chromatographic determination of less polar ginsenosides in processed ginseng. J. Chromatogr. 2001; 921: 335-339   DOI   ScienceOn