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Biosafety of Microbiological Laboratories in Korea  

Eun, Sang-Jun (Department of Health Policy and Management, Seoul National University College of Medicine)
Park, Ki-Dong (Division of Communicable Disease Control, Korea Center for Disease Control and Prevention)
Kim, Jong-Kyun (Department of Preventive Medicine, Gachon Medical School)
Im, Jeong-Soo (Department of Preventive Medicine, Gachon Medical School)
Hwang, Yoo-Sung (Neodin Medical Institute)
Kim, Yong-Ik (Department of Health Policy and Management, Seoul National University College of Medicine)
Lee, Jin-Yong (Department of Health Policy and Management, Seoul National University College of Medicine)
Publication Information
Journal of Preventive Medicine and Public Health / v.38, no.4, 2005 , pp. 449-456 More about this Journal
Abstract
Objectives : The biosafety level (BSL) practiced in microbiology laboratories in Korea according to the laboratory biosafety manual published by the World Health Organization (WHO) was evaluated using the data obtained by a survey. Methods : Under the advise of Clinical Laboratory Physicians, 144 types of microorganisms were screened based on the guidelines of biosafety in microbiological and biomedical laboratories published by the US Center for Disease Control and Prevention and classified into 1-4 risk groups. A questionnaire containing 21 questions in 5 areas was developed using the biosafety manual by published WHO. Of the 1,876 different organizations sent the survey, 563 responded to the survey (response rate: 30.0%). The species of microoganisms handled by as well as the biosafety level in microbiology laboratories were analyzed. Results : There were 123 species of microorganisms handled in microbiology labs in Korea. The BSL required in 512 microbiology labs was answered by the survey responders as the first grade in 33 labs (6.4%), 2nd in 437 (85.4%), 3rd in 42 (8.2%), and 4th in none. The average number of items satisfied was 12.2, showing only a 57.9% satisfactory rate and normal distribution. Conclusions : The state of overall observance of BSL in most microbiology labs of Korea was evaluated as lagging compared with the standard set up by WHO. Therefore, the Korean government need to produce and distribute a biosafety manual in microbiology laboratories and make efforts to prevent this threat through measures such as training in biosafety in microbiology labs.
Keywords
Safety; Laboratory Infection;
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  • Reference
1 Grist NR. Hepatitis in clinical laboratories a three year survey. J Clin Pathol 1975; 28: 255-259   DOI   PUBMED
2 Kim JS, Lee HW. Studies on microbiololgical laboratory biosafety (1) a survey on biosafety status in laboratory department of training. Report NIH Korea 1986; 23: 139-160 (Korean)
3 보건복지부. 유전자재조합실험지침 보건복지부고시 제1997-22호. 보건복지부; 1996
4 Sulkin SE, Pike RM. Survey of laboratoryacquired infections. Am J Public Health 1951; 41(7): 769-781   DOI
5 Sung WK. Development of the system ensuring biosafety in genetic modification experiment. Health and Welfare Policy Forum 2001; 4: 45-58 (Korean)
6 Office of Laboratory Security. The laboratory biosafety guidelines. 3th edition(draft).Ottawa. 2001
7 Advisory Committee on Dangerous Pathogens. Categorization of biological agents accordingto hazard and categories of containment. 4th edition. London. 1995
8 Minor PD. Biosafety consequences of eradication of wild-type polioviruses. Lancet 2001; 358(9277): 166-168   DOI   ScienceOn
9 Orellana C. Laboratory-acquired SARS raises worries on biosafety. Lancet Infect Dis 2004; 4(2): 64
10 CDC and NIH. Biosafety in microbiological and biomedical laboratories. 4th edition.Washington. 1999
11 Holmes GP, Hilliard JK, KLontz KC, Rupert AH, Schindler CM, Parrish E, Griffin DG, Ward GS, Bernstein ND, Bean TW, Ball MR, Bready JA, Wilder MH, Kaplan JE. B virus(Herpesvirus simiae) infection in humans: epidemiologic investigation of a cluster. Ann Int Med 1990; 112: 833-839   DOI   ScienceOn
12 Grist NR. Hepatitis in clinical laboratories 1973-1974. J Clin Pathol 1976; 29: 480-483   DOI   PUBMED
13 Wehrle PF. Smallpox eradication. A global appraisal.JAMA 1978; 240(18): 1977-1979   DOI   PUBMED   ScienceOn
14 Grist NR. Hepatitis in clinical laboratories 1975-1976. J Clin Pathol 1978; 31: 415-417   DOI   PUBMED
15 Her majesty's stationary office. Report of the committee of inquiry into smallpox outbreakin London in march and April 1973. London. 1974
16 Cho SH, Ju YS, Kang D, Kim S, Kim IS, Hong ST. Laboratory-acquired infection with hantavirus at a research unit of medical school in Seoul, 1996. Korean J Prev Med 1999; 32(3): 269-275 (Korean)
17 Richrdson JH. Provisional summary of 109 laboratory-associated infections at the Center for Disease Control, 1947-1973. Presented at the 16th Annual Biosafety Conference, Ames, Iowa. 1973
18 WHO. Laboratory biosafety mannual. 2nd edition(revised). Geneva. 2003
19 World Health Organization. Smallpox surveillance. Weekly Epidemiol Record 1978; 53(35): 265-266
20 김용익, 박기동, 임정수, 황유성, 생물테러 가능 병원체와 실험실에 대한 안전등급제 도입 및 운영방안 개발. 국립보권원. 생물 테러대비 두창백신 희석 활용 및 집단예방 접종 방안 개발. 2003
21 Lee HW, Johnson KM. Laboratory-acquired infections with hantaan virus, the etiologicagent of Korean hemmorrhagic fever. J Infec Dis 1982; 146; 645-651   DOI   PUBMED   ScienceOn
22 남명진. 안전관련 법령 제정 방안. 이의경. 생명과학관련 국민보건안전윤리 확보방안. 2000년 보건의료기술개발사업 정책연구개발사업보고서. 보건복지부 2001
23 Oliphant JW, Parker RR. Q fever: Three cases of laboratory infection. Public Health Rep 1948; 63(42): 1364-1370   DOI   PUBMED
24 Beeman EA. Q fever-An epidemiological note. Public Health Rep 1950; 65(2): 88-92   DOI   PUBMED   ScienceOn
25 Kissalt A. Laboratoriums infektionen mit typhusbazillen. Zeit Hyg Infectionkrank 1915; 80: 145-162   DOI
26 보건복지부. 전염병병원체 검사. 보존 및 관리 지침, 보건복지부 고시 2001-25호. 보건복지부; 2001
27 Meyer KF, Eddie B. Laboratory infections due to brucella. J Infect Dis 1941; 68: 24-32   DOI
28 Reid DD. Incidence of tuberculosis among workers in medical laboratories. Br Med J 1957; 2: 10-14   DOI
29 Martini GA, Schmidt HA. Spermatogenic transmission of Marburg viruus. Klin Wschr 1968; 46: 398-400   DOI   PUBMED