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http://dx.doi.org/10.4110/in.2015.15.3.161

Early Growth Response-1 Plays a Non-redundant Role in the Differentiation of B Cells into Plasma Cells  

Oh, Yeon-Kyung (Laboratory of Autoimmunology, Department of Anatomy and Cell Biology, College of Medicine, Hanyang University)
Jang, Eunkyeong (Laboratory of Autoimmunology, Department of Anatomy and Cell Biology, College of Medicine, Hanyang University)
Paik, Doo-Jin (Laboratory of Autoimmunology, Department of Anatomy and Cell Biology, College of Medicine, Hanyang University)
Youn, Jeehee (Laboratory of Autoimmunology, Department of Anatomy and Cell Biology, College of Medicine, Hanyang University)
Publication Information
IMMUNE NETWORK / v.15, no.3, 2015 , pp. 161-166 More about this Journal
Abstract
Early growth response (Egr)-1 is a $Cys_2-His_2-type$ zincfinger transcription factor. It has been shown to induce survival and proliferation of immature and mature B cells, respectively, but its role in the differentiation of B cells into plasma cells remains unclear. To examine the effects of Egr-1 deficiency on the activation of B cells, naive B cells from $Egr1^{-/-}$mice and their wild-type (WT) littermates were activated to proliferate and differentiate, and then assayed by FACS. Proportions of cells undergoing proliferation and apoptosis did not differ between $Egr1^{-/-}$ and WT mice. However, $Egr1^{-/-}$ B cells gave rise to fewer plasma cells than WT B cells. Consistently, $Egr1^{-/-}$ mice produced significantly lower titer of antigen-specific IgG than their WT littermates upon immunization. Our results demonstrate that Egr-1 participates in the differentiation program of B cells into plasma cells, while it is dispensable for the proliferation and survival of mature B cells.
Keywords
Egr1; B cells; Plasma cells; Differentiation; Antibody;
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