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http://dx.doi.org/10.4110/in.2015.15.2.100

1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (EC-18) Modulates Th2 Immunity through Attenuation of IL-4 Expression  

Yoon, Sun Young (ENZYCHEM Lifesciences)
Kang, Ho Bum (Biomedical Translational Research Center, Korea Research Institute of Bioscience and Biotechnology)
Ko, Young-Eun (Biomedical Translational Research Center, Korea Research Institute of Bioscience and Biotechnology)
Shin, Su-Hyun (Biomedical Translational Research Center, Korea Research Institute of Bioscience and Biotechnology)
Kim, Young-Jun (Biomedical Translational Research Center, Korea Research Institute of Bioscience and Biotechnology)
Sohn, Ki-Young (ENZYCHEM Lifesciences)
Han, Yong-Hae (ENZYCHEM Lifesciences)
Chong, Saeho (ENZYCHEM Lifesciences)
Kim, Jae Wha (Biomedical Translational Research Center, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
IMMUNE NETWORK / v.15, no.2, 2015 , pp. 100-109 More about this Journal
Abstract
Controlling balance between T-helper type 1 (Th1) and T-helper type 2 (Th2) plays a pivotal role in maintaining the biological rhythm of Th1/Th2 and circumventing diseases caused by Th1/Th2 imbalance. Interleukin 4 (IL-4) is a Th2-type cytokine and often associated with hypersensitivity-related diseases such as atopic dermatitis and allergies when overexpressed. In this study, we have tried to elucidate the function of 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (EC-18) as an essential modulator of Th1/Th2 balance. EC-18 has showed an inhibitory effect on the production of IL-4 in a dose-dependent manner. RT-PCR analysis has proved EC-18 affect the transcription of IL-4. By analyzing the phosphorylation status of Signal transducer and activator of transcription 6 (STAT6), which is a transcriptional activator of IL-4 expression, we discovered that EC-18 induced the decrease of STAT6 activity in several stimulated cell lines, which was also showed in STAT6 reporter analysis. Co-treatment of EC-18 significantly weakened atopy-like phenotypes in mice treated with an allergen. Collectively, our results suggest that EC-18 is a potent Th2 modulating factor by regulating the transcription of IL-4 via STAT6 modulation, and could be developed for immune-modulatory therapeutics.
Keywords
EC-18; Th2 modulator; IL-4; STAT6;
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