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http://dx.doi.org/10.4110/in.2012.12.6.277

Vitamin C Up-regulates Expression of CD80, CD86 and MHC Class II on Dendritic Cell Line, DC-1 Via the Activation of p38 MAPK  

Kim, Hyung Woo (School of Korean Medicine, Pusan National University)
Cho, Su In (School of Korean Medicine, Pusan National University)
Bae, Seyeon (Laboratory of Immunology and Vitamin C, Department of Anatomy, Seoul National University College of Medicine)
Kim, Hyemin (Laboratory of Immunology and Vitamin C, Department of Anatomy, Seoul National University College of Medicine)
Kim, Yejin (Laboratory of Immunology and Vitamin C, Department of Anatomy, Seoul National University College of Medicine)
Hwang, Young-Il (Laboratory of Immunology and Vitamin C, Department of Anatomy, Seoul National University College of Medicine)
Kang, Jae Seung (Laboratory of Immunology and Vitamin C, Department of Anatomy, Seoul National University College of Medicine)
Lee, Wang Jae (Laboratory of Immunology and Vitamin C, Department of Anatomy, Seoul National University College of Medicine)
Publication Information
IMMUNE NETWORK / v.12, no.6, 2012 , pp. 277-283 More about this Journal
Abstract
Vitamin C is an essential water-soluble nutrient which primarily exerts its effect on host defense mechanisms and immune homeostasis, but the mechanism related to immune-potentiation is poorly understood. Since dendritic cells (DCs) are known as a potent antigen presenting cell (APC) that could enhance the antigen specific immune responses, we investigate the effects of vitamin C on activation of DCs and its related mechanism by using dendritic cell lines, DC-1. First, we found that there was no damage on DC-1 by 2.5 mM of vitamin C. In the presence of vitamin C, the expression of CD80, CD86, and MHC molecules was increased, but it was decreased by the pre-treatment of SB203580, p38 MAPK-specific inhibitor. We confirmed the phosphorylation of p38 MAPK was increased by the treatment of vitamin C. Taken together, these results suggest that vitamin C could enhance the activity of dendritic cells via the up-regulation of the expression of CD80, CD86, and MHC molecules and the activation of p38 MAPK is related to this process.
Keywords
Vitamin C; Dendritic cell; CD80; CD86; MHC class II; p38 MAPK;
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