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http://dx.doi.org/10.4110/in.2012.12.5.207

T Cell Immunoglobulin Mucin Domain (TIM)-3 Promoter Activity in a Human Mast Cell Line  

Kim, Jung Sik (Department of Microbiology, Ajou University School of Medicine)
Shin, Dong-Chul (Department of Microbiology, Ajou University School of Medicine)
Woo, Min-Yeong (Department of Microbiology, Ajou University School of Medicine)
Kwon, Myung-Hee (Department of Microbiology, Ajou University School of Medicine)
Kim, Kyongmin (Department of Microbiology, Ajou University School of Medicine)
Park, Sun (Department of Microbiology, Ajou University School of Medicine)
Publication Information
IMMUNE NETWORK / v.12, no.5, 2012 , pp. 207-212 More about this Journal
Abstract
T cell immunoglobulin mucin domain (TIM)-3 is an immunomodulatory molecule and upregulated in T cells by several cytokines. TIM-3 also influences mast cell function but its transcriptional regulation in mast cells has not been clarified. Therefore, we examined the transcript level and the promoter activity of TIM-3 in mast cells. The TIM-3 transcript level was assessed by real-time RT-PCR and promoter activity by luciferase reporter assay. TIM-3 mRNA levels were increased in HMC-1, a human mast cell line by TGF-${\beta}1$ stimulation but not by stimulation with interferon (IFN)-${\alpha}$, IFN-${\lambda}$, TNF-${\alpha}$, or IL-10. TIM-3 promoter -349~+144 bp region relative to the transcription start site was crucial for the basal and TGF-${\beta}1$-induced TIM-3 promoter activities in HMC-1 cells. TIM-3 promoter activity was increased by over-expression of Smad2 and Smad4, downstream molecules of TGF-${\beta}1$ signaling. Our results localize TIM-3 promoter activity to the region spanning -349 to ＋144 bp in resting and TGF-${\beta}1$ stimulated mast cells.
Keywords
T cell immunoglobulin mucin domain-3; TGF-${\beta}1$; Transcription; Mast cells; Smad;
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