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http://dx.doi.org/10.4110/in.2011.11.6.416

Th17 Responses Are not Induced in Dextran Sodium Sulfate Model of Acute Colitis  

Kim, Yoon-Suk (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University at Wonju)
Lee, Min-Ho (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University at Wonju)
Ju, Ahn-Seung (Department of Biomedical Laboratory Science, Daegu Health College)
Rhee, Ki-Jong (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University at Wonju)
Publication Information
IMMUNE NETWORK / v.11, no.6, 2011 , pp. 416-419 More about this Journal
Abstract
Dextran sodium sulfate (DSS) is a widely used chemical model for inflammatory bowel disease (IBD). It is thought that imbalances in the T helper (Th) cell subsets contribute to IBD. Recent studies suggest that the acute DSS-colitis model is polarized toward a Th1/Th17 profile based on RT-PCR analysis of colonic tissues. In the current study we determined whether colonic Th cells from DSS-colitis mice were skewed toward the Th17 profile. Mice were treated with 5% DSS for 7 days and colonic T cells isolated and examined for production of IFN-${\gamma}$ (Th1 cell), IL-4 (Th2 cell) and IL-17 (Th17 cell) by intracellular flow cytometry. We found that the percentage of colonic Th17 cells were similar to non-treated controls but the percentage of Th1 cells were elevated in DSS-colitis mice. These results suggest that in the acute DSS-colitis model the colonic Th cells exhibit a Th1 profile and not a Th17 profile.
Keywords
Dextran sodium sulfate; Inflammatory bowel disease; Th17 cell; Colitis;
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