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http://dx.doi.org/10.4110/in.2010.10.4.126

Expression of Hepatitis B Virus X Protein in Hepatocytes Suppresses CD8+ T Cell Activity  

Lee, Mi Jin (Department of Microbiology and Immunology, Ajou University School of Medicine)
Jin, Young-hee (Department of Microbiology and Immunology, Ajou University School of Medicine)
Kim, Kyongmin (Department of Microbiology and Immunology, Ajou University School of Medicine)
Choi, Yangkyu (Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University)
Kim, Hyoung-Chin (Biomedical Mouse Resource Center, Korea Research Institute of Bioscience and Biotechnology)
Park, Sun (Department of Microbiology and Immunology, Ajou University School of Medicine)
Publication Information
IMMUNE NETWORK / v.10, no.4, 2010 , pp. 126-134 More about this Journal
Abstract
Background: $CD8^+$ T cells contribute to the clearance of Hepatitis B virus (HBV) infection and an insufficient $CD8^+$ T cell response may be one of the major factors leading to chronic HBV infection. Since the HBx antigen of HBV can up-regulate cellular expression of several immunomodulatory molecules, we hypothesized that HBx expression in hepatocytes might affect $CD8^+$ T cell activity. Methods: We analyzed the activation and apoptosis of $CD8^+$ T cells co-cultured with primary hepatocytes rendered capable of expressing HBx by recombinant baculovirus infection. Results: Expression of HBx in hepatocytes induced low production of $interferon-{\gamma}$ and apoptosis of CD8+ T cells, with no effect on CD8 T cell proliferation. However, transcriptional levels of H-2K, ICAM-1 and PD-1 ligand did not correlate with HBx expression in hepatocytes. Conclusion: Our results suggest that HBx may inhibit $CD8^+$ T cell response by regulation of $interferon-{\gamma}$ production and apoptosis.
Keywords
Hepadnaviridae; T-lymphocytes; Cytotoxic; Viral proteins; Apoptosis; $interferon-{\gamma}$;
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