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http://dx.doi.org/10.4110/in.2009.9.5.179

Prophylactic and Therapeutic Potential of Asp f1 Epitopes in Naive and Sensitized BALB/c Mice  

Chaudhary, Neelkamal (Institute of Genomics and Integrative Biology)
Mahajan, Lakshna (Institute of Genomics and Integrative Biology)
Madan, Taruna (Institute of Genomics and Integrative Biology)
Kumar, Anil (School of Biotechnology, Devi Ahilya Vishwavidyalaya)
Raghava, Gajendra Pratap Singh (Institute of Microbial Technology)
Katti, Seturam Bandacharya (Biopolymers Division, Central Drug Research Institute)
Haq, Wahajul (Biopolymers Division, Central Drug Research Institute)
Sarma, Puranam Usha (Institute of Genomics and Integrative Biology)
Publication Information
IMMUNE NETWORK / v.9, no.5, 2009 , pp. 179-191 More about this Journal
Abstract
Background: The present study examines a hypothesis that short allergen-derived peptides may shift an Aspergillus fumigatus (Afu-) specific TH2 response towards a protective TH1. Five overlapping peptides (P1-P5) derived from Asp f1, a major allergen/antigen of Afu, were evaluated for prophylactic or therapeutic efficacy in BALB/c mice. Methods: To evaluate the prophylactic efficacy, peptides were intranasally administered to naive mice and challenged with Afu-allergens/antigens. For evaluation of therapeutic efficacy, the mice were sensitized with Afu-allergens/antigens followed by intranasal administration of peptides. The groups were compared for the levels of Afu-specific antibodies in sera and splenic cytokines evaluated by ELISA. Eosinophil peroxidase activity was examined in the lung cell suspensions and lung inflammation was assessed by histopathogy. Results: Peptides P1-, P2- and P3 decreased Afu-specific IgE (84.5~98.9%) and IgG antibodies (45.7~71.6%) in comparison with Afu-sensitized mice prophylactically. P1- and P2-treated ABPA mice showed decline in Afu-specific IgE (76.4~88%) and IgG antibodies (15~54%). Increased IgG2a/IgG1 and IFN-${\gamma}$/IL-4 ratios were observed. P1-P3 prophylactically and P1 therapeutically decreased IL-5 levels and eosinophil peroxidase activity. P1 decreased inflammatory cells' infiltration in lung tissue comparable to non-challenged control. Conclusion: Asp f1-derived peptide P1, prophylactically and therapeutically administered to Balb/c mice, is effective in regulating allergic response to allergens/antigens of Afu, and may be explored for immunotherapy of allergic aspergillosis in humans.
Keywords
Aspergillus fumigatus; allergic bronchopulmonary aspergillosis; Asp f1 peptides; cytokines; eosinophil; T helper cell;
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