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http://dx.doi.org/10.4110/in.2009.9.5.169

Enhancement of DNA Vaccine-induced Immune Responses by Influenza Virus NP Gene  

Choi, So-Young (Research Institute, Genexine Co. Ltd.)
Suh, You-Suk (Research Institute, Genexine Co. Ltd.)
Cho, Jae-Ho (Garvan Institute of Medical Research)
Jin, Hyun-Tak (Research Institute, Genexine Co. Ltd.)
Chang, Jun (Division of Life and Pharmaceutical Sciences, and Center for Cell signaling & Drug Discovery Research, Ewha Womans University)
Sung, Young-Chul (Research Institute, Genexine Co. Ltd.)
Publication Information
IMMUNE NETWORK / v.9, no.5, 2009 , pp. 169-178 More about this Journal
Abstract
DNA immunization induces B and T cell responses to various pathogens and tumors. However, these responses are known to be relatively weak and often transient. Thus, novel strategies are necessary for enhancing immune responses induced by DNA immunization. Here, we demonstrated that co-immunization of influenza virus nucleoprotein (NP) gene significantly enhances humoral and cell-mediated responses to codelivered antigens in mice. We also found that NP DNA coimmunization augments in vivo proliferation of adoptively transferred antigen-specific CD4 and CD8 T cells, which enhanced protective immunity against tumor challenge. Our results suggest that NP DNA can serve as a novel genetic adjuvant in cocktail DNA vaccination.
Keywords
DNA vaccine; influenza virus NP; adjuvant;
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