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http://dx.doi.org/10.4110/in.2009.9.4.127

Expression and Function of TLR2 on CD4 Versus CD8 T Cells  

Lee, Sun-Mi (Departments of Microbiology and Immunology, College of Medicine, Inje University)
Joo, Young-Don (Departments of Hemato/Oncology, College of Medicine, Inje University)
Seo, Su-Kil (Departments of Microbiology and Immunology, College of Medicine, Inje University)
Publication Information
IMMUNE NETWORK / v.9, no.4, 2009 , pp. 127-132 More about this Journal
Abstract
Background: Toll-like receptors (TLRs) play a fundamental role in innate immunity through their capacity to recognize pathogen-associated molecular patterns. Also, TLRs that are expressed in T cells are reported to function as co-stimulatory receptors. However, the functional capacity of TLRs on CD4 T and CD8 T cells has not been directly compared. Here we compared CD4 and CD8 T cell responses to TLR2 ligand plus TCR-mediated stimulation. Methods: TLR2 expression was analyzed on T cell subsets under naive and alloantigen-primed conditions. We analyzed the effects of TLR2 co-stimulation on proliferation and survival of T cell subsets in vitro when stimulated with soluble anti-CD3 in the presence or absence of synthetic ligand $Pam_3CSK_4$. Results: TLR2 expression on CD8 T cells was induced following activation; this expression was much higher than on CD4 T cells. Thus, the molecule was constitutively expressed on Listeriaspecific memory CD8 T cells. Based on these expression levels, proliferation and survival were markedly elevated in CD8 T cells in response to the TLR2 co-stimulation by $Pam_3CSK_4$ compared with those in CD4 T cells. Conclusion: Our data show that TLR2 co-stimulation is more responsible for proliferation and survival of CD8 T cells than for that of CD4 T cells.
Keywords
TLR2; T cell co-stimulation; CD4 T cell; CD8 T cell;
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