Browse > Article

Effect of Dexamethasone on the Surface Expression of Marker Molecules and Differentiation of Murine B Cells  

Yeo, Seung-Geun (Department of Otorhinolaryngology, Head and Neck Surgery, College of Medicine, Kyung Hee University)
Cha, Chang-Il (Department of Otorhinolaryngology, Head and Neck Surgery, College of Medicine, Kyung Hee University)
Park, Dong-Choon (Department of Obstetric and Gynecology, College of Medicine, The Catholic University of Korea)
Publication Information
IMMUNE NETWORK / v.6, no.3, 2006 , pp. 138-144 More about this Journal
Abstract
Background: There are at least two different subsets of B cells, B-1 and B-2. The characteristic features and function of B-2 cells in addition to the effect of steroids on B-2 cells are well-known. Although B-1 cells have different features and functions from B-2 cells, the effect of steroids on B-1 cells is not completely understood. Therefore, this study examined the effects of dexamethasone on peritoneal (or B-1 cells) and splenic B cells (or B-2 cells). Methods: Purified B cells were obtained from the peritoneal fluid and the spleens of mice. The isolated B cells were cultured in a medium and after adding different concentrations of dexamaethasone. The cell survival rate was measured by flow cytometry using propidium iodide. The expression level of the B cell surface marker was analyzed by flow cytometry. During the culture of these cells, immunoglobulin secreted into the culture supernatants was evaluated by an enzyme-linked immunosorbent assay. Results: The survival rate of peritoneal and splenic B cells decreased with increasing dexamethasone concentration. However, the rate of peritofieal B cell apoptosis was lower than that of splenic B cells. CDS and B7.1 expression in peritoneal B cells and CD23 and sIgM expression in splenic B cells after the dexamethasone treatment were reduced. When B cells were treated with dexamethasone, the spontaneous IgM secretion decreased with increasing dexamethasone concentration. Conclusion: Dexamethasone induces apoptosis in peritoneal and splenic B cells. However, peritoneal B cells are less sensitive to dexamethasone. The dexamethasone suppressed expression of the surface markers in peritoneal B cells is different from those in splenic B cells.
Keywords
Dexamethasone; peritoneal B cell; splenic B cell;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Rothstein TL: Cutting edge commentary: two B-1 or not to be one. J Immunol 168;4257-4261, 2002   DOI
2 Andreau K, Lemaire C, Souvannavong V, Adam A: Induction of apoptosis by dexamethasone in the B cell lineage. Immunopharmacol 40;67-76, 1998   DOI   ScienceOn
3 Fischer A, Konig W: Regulation of CD23 expression, soluble CD23 release and immunoglobulin synthesis of peripheral blood lymphocytes by glucocorticoids. Immunol 71;473-479, 1990
4 Metcalf D: Cortisone action on serum colony-stimulating factor and bone marrow in vitro colony-forming cells. Proc Soc Exp Biol Med 132;391-394, 1969
5 Thompson J, Van Furth R: The effect of glucocorticosteroids on the proliferation and cinetics of promonocytes and monocytes of the bone marrow. J Exp Med 137;10-21, 1973   DOI   ScienceOn
6 Roess DA, Bellone CJ, Ruh NIF, Nadel EM, Ruh TS: The effect of glucocorticoids on mitogen-stimulated B lymphocytes: thymidine incorporation and antibody secretion Endocrinology 11 0;169-175, 1982   DOI   ScienceOn
7 Andersson J, Moller G, Sjoberg O: Induction of immunoglobulin and antibody synthesis in vitro by lipopolysaccharide. Eur J Immunol 2;349-353, 1972   DOI   ScienceOn
8 Adcock IM, Caramori G, Ito K: New insights into the molecular mechanisms of corticosteroids actions. Curr Drug Targets 7;649-660, 2006   DOI   ScienceOn
9 Sabbele NR, Oudenaren AV, Hooijkaas H, Benner R: The effect of corticosteroids upon murine B cells in vivo and in vitro as determined in the LPS-culture system. Immunol 62;285-290, 1987
10 Pene J. Rousset F, Briere F, Chretien I, Wideman J, Bonnefoy JY: Interleukin 5 enhances interleukin 4-induced IgE production by normal human B cells. The role of soluble CD23 antigen. Eur J Immunol 18;929-935, 1998   DOI   ScienceOn
11 Barnes PJ: Corticosteroids, IgE, and atopy. J Clin Invest 107;265-266, 2001   DOI   ScienceOn
12 Bach JF, Strom TB: Corticosteroids. In: Turked 11, ed.: The mode of action of immunosuppressive agents (research monographs in immunology), p21-104, Amsterdam Elsevier, 1985
13 Jirapongsananuruk O, Leung DYM: The modulation of B7.2 and B7.1 on B cells by immunosuppressive agents. Clin Exp Immunol 118;1-8,1999
14 Yeo SG, Cho Js, Park DC, Rothstein TL: B-1 cells differ from conventional B (B-2) cells: difference in proliferation. Immune Network 4;155-160, 2004   DOI
15 Claman HN: Corticosteroids and lymphoid cells. N Engl J Med 287;388-397, 1972   DOI   ScienceOn
16 Brahim F: Marrow lymphocyte production during chronic hydrocortisone administration. J Reticuloendothel Soc 23; 111-118, 1978
17 Barnes PJ: Anti-inflammatory actions of glucocorticoids: molecular mechanisms. Clin Sci 94;557-572, 1998   DOI
18 Kim JB, Yeo SG, Kim SW, Cho Js, Cha CI: Characteristic features of immune B cells in murine cervical lymph node. Korean J Otolaryngol 48;241-246, 2005
19 Pozzato G, de Paoli P, Franzin F, Tulissi P, Moretti M, Basaglia G: Effects of alpha-interferon and steroids on CD23 expression and release in B-cell chronic lymphocytic leukemia. Haematologica 79;205-212, 1994
20 Barnes PJ: Anti-inflammatory actions of glucocorticoids: molecular mechanisms. Clinical Science 94;557-572, 1998   DOI