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Differences in Their Proliferation and Differentiation between B-1 and B-2 Cell  

Yeo, Seung-Geun (Department of Otolaryngology, College of Medicine, Kyung Hee University)
Cha, Chang-Il (Department of Otolaryngology, College of Medicine, Kyung Hee University)
Park, Dong-Choon (Department of Obstetric and Gynecology, St. Vincent's Hospital, The Catholic University of Korea)
Publication Information
IMMUNE NETWORK / v.6, no.1, 2006 , pp. 1-5 More about this Journal
Abstract
Background: B cell subset has been divided into B-1 cells and B-2 cells. B-1 cells are found most prominently in the peritoneal cavity, as well as constituting a small pro portion of splenic B cells and they are larger and less dense than B-2 cells in morphology. This study was designed to compare the differences in their proliferation and differentiation between B-1 and B-2 cell. Methods: We obtained sorted B-1 cells from peritoneal fluid and B-2 cells from spleens of mice. Secreted IgM was measured by enzyme-linked immunosorbent assay. Entering of S phase in response to LPS-stimuli was measured by proliferative assay. Cell cycle analysis by propidium iodide was performed. p21 expression was assessed by real time PCR. Results: Cell proliferation and cell cycle progression in B-1 and B-2 cells, which did not occur in the absence of LPS, required LPS stimulation. After LPS stimulation, B-1 and B-2 cells were shifted to Sand G2/M phases. p21 expression by resting B-1 cells was higher than that of resting B-2 cells. Conclusion: B-1 cells differ from conventional B-2 cells in proliferation, differentiation and cell cycle.
Keywords
B-1 cell; B-2 cell; immunology; differentiation; cell cycle; p21;
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