Browse > Article

Inhibition of Major Histocompatibility Complex (MHC)-Restricted Presentation of Exogenous Antigen in Dendritic Cells by Korean Propolis Components  

Han, Shin-Ha (Department of Biology, Seoul Women's University)
Cho, Kyung-Hae (Department of Biology, Seoul Women's University)
Lee, Seung-Jeong (College of Pharmacy, Chungbuk National University)
Lee, Chong-Kil (College of Pharmacy, Chungbuk National University)
Song, Young-Cheon (Department of Pharmacy, Sahmyook University)
Ha, Nam-Joo (Department of Pharmacy, Sahmyook University)
Kim, Kyung-Jae (Department of Pharmacy, Sahmyook University)
Publication Information
IMMUNE NETWORK / v.5, no.3, 2005 , pp. 150-156 More about this Journal
Abstract
Background: Dendritic cells (DCs) playa critical role not only in the initiation of immune responses, but also in the induction of immune tolerance. In an effort to regulate immune responses through the modulation of antigen presenting cell (APC) function of DCs, we searched for and characterized APC function modulators from natural products. Methods: DCs were cultured in the presence of propolis components, WP and CP, and then examined for their ability to present exogenous antigen in association with major histocompatibility complexes (MHC). Results: WP and CP inhibited class I MHC-restricted presentation of exogenous antigen (cross-presentation) in a DC cell line, DC2.4 cells, and DCs generated from bone marrow cells with GM-CSF and IL-4. The inhibitory activity of WP and CP appeared to be due not only to inhibition of phagocytic activity of DCs, but also to suppression of expression of MHC molecules on DCs. We also examined the effects of WP and CP on T cells. Interestingly, WP and CP increased IL-2 production from T cells. Conclusion: These results demonstrate that WP and CP inhibit MHC-restricted presentation of exogenous antigen through down-regulation of phagocytic activity and suppression of expression of MHC molecules on DCs.
Keywords
Propolis; dendritic cell; major histocompatibility complex; antigen presentation;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Celluzzi CM, Mayordomo JI, Storkus WJ, Lotze MT, LD Jr: Peptide-pulsed dendritic cells induce antizen-speci CTL-mediated protective tumor immunity. J Exp Med 183;283-287, 1996   DOI   ScienceOn
2 Shurin MR: Dendritic cells presenting tumor antigen. Can Immunol Immunother 43;158-164, 1996   DOI   ScienceOn
3 Fernandez NC, Lozier A, Flament C, Ricciardi-Castagno Bellet D, Suter M, Perricaudet M, Tursz T, Maraskovskv Zirvogel L: Dendritic cells directly trigger NK cell functio Cross-talk relevant in innate anti-tumor immune response vivo. Nature Medicine 5;405-411, 1999   DOI   ScienceOn
4 Han S, Sung KH, Yim D, Lee S, Cho K, Lee CK, Ha N Kim K: Activation of murine macrophage cell line RA 264.7 by Korean Propolis. Arch Pharm Res 25:895-902, 2002   DOI   ScienceOn
5 Harborne JB, Williams CA: Advances in flavonoid resea since 1992. Phytochemistry. 55;481-504, 2000   DOI   ScienceOn
6 Shen Z, Reznikoff G, Dranoff G, Rock KL: Cloned dendr cells can present exogenous antigens on both MHC clas and class II molecules. J Immunol 158;2723-2730, 1977
7 Larsson M, Fonteneau JF, Bhardwaj N: Dendritic cells surrect antigens from dead cells. Trends Immunol 22;14 148, 2001
8 Yewdell JW, Norbury CC, Bennink JR: Mechanisms of ogenous antigen presentation by MHC class I molecules vitro and in vivo: implications for generating CD8+ T responses to infectious agents, tumors, transplants, and v cines. Adv Immunol 73;1-77, 1999   DOI   PUBMED
9 Inaba K. Young JW, Steinman RM: Direct activation of CD cytotoxic T lymphocytes by dendritic cells. J Exp Med 166; 182-194, 1987   DOI   ScienceOn
10 Muller L, Provenzani C. Faul C. Pawelec G: Recognition chronic myelogenous leukemia cells by autologous T lymp cytes primed in vitro against the patient's dendritic cells. Br J Hematol 112;740-748, 2001   DOI   ScienceOn
11 Flamand V, Sornasse T, Thielemans K, Demanet C, Bak M, Bazin H, Tielemans F, Leo O, Urbain J. Moser M: Mu dendritic cells pulsed in vitro with tumor antigen induce mor resistance in vivo. Eur J Immunol 24;605-610, 1994   DOI   ScienceOn
12 Shen Z, Reznikoff G, Dranoff G, Rock KL: Cloned dendr cells can present exogenous antigens on both MHC clas and class II molecules. J Immunol 15;2723-2730, 1997
13 Lee JK, Lee MK, Yun YP: Acemannan purified from A vera induces phenotypic and functional maturation of im ture dendritic cells. Int Immunopharmacol 1:1275-1284, 2001   DOI   ScienceOn
14 Ivanovsca ND, Dimov VD, Pavlova S, Bankova VS, Pop SS, Immunomodulatorv action of propolis. VI. Anticom mentary activity of a water-soluble derivative. J Ethnophar macology 47;135-143, 1995   DOI
15 Fields RC, Shimizu K, Mule JJ: Murine dendritic cells pu with whole tumor lysates mediate potent antitumor imm responses in vitro and in vivo. Proc Natl Acad Sci 95;9482-9487, 1998
16 Mayordomo JI, Zorina T, Storkus WJ. Zitvogel L, Cell C. Falo LD, Melief CJ. Ildstad ST, Kast WM, Deleo A: Bo marrow-derived dendritic cells pulsed with synthetic tum peotides elicit protective and therapeutic antitumour imm nity. Nat Med 1:1297-1302, 1995   DOI   ScienceOn
17 Moron G, Dadaclio G, Leclerc C: New tools for antig delivery to the MHC class I pathway. Trends Immunol 25;92-97, 2004   DOI   ScienceOn
18 Lee YR, Yang IH, Lee YH, Im SA, Song S, Li H, Han Kim K, Eo SK, Lee CK: Cyclosporin A and tacrolimus, not rapamycin, inhibit MHC-restricted antigen presenta pathways in dendritic cells. Blood 105;3951-3955, 2005   DOI   ScienceOn
19 Steinman RM, Inaba K, Turley S, Pierre P, Mellman I: tigen capture, processing, and presentation by dendritic ce recent cell biological studies. Hum Immunol 60;562-5 1999   DOI   ScienceOn
20 Avigan DD: Dendritic cells: development, function and tential use for cancer immunotherapy. Blood Reviews 13;51-64, 1999   DOI   PUBMED   ScienceOn
21 Gong J. Chen D, Kashiwaba M, Kufe D: Induction of anti mor activity by immunization with fusions of dendritic carcinoma cells. Nat Med 3:558-561, 1997   DOI   ScienceOn
22 Inaba K. Metlay JP, Crowley MT, Steinman RM: Dendritic pulsed with protein antigens in vitro can prime antigen-spec MHGrestricted T cells in situ. J Exp Med 172;631-640, 1990   DOI   ScienceOn
23 Fong L, Engleman EG: Dendritic cells in cancer immunot rapy. Annul Rev Immunol 18;245-273, 2000   DOI   ScienceOn
24 Hart DN: Dendritic cells: unique leukocyte populations, w control the primary immune response. Blood 90;3245-3287, 1997
25 Harding CV, Collins D, Kanagawa O, Unanue ER: Lipo me-encapsulated antigens engender lysosomal processing f class II MHC presentation and cvtosolic processing for cl I presentation. J Immunol 147;2860-2863, 1991   PUBMED
26 Inaba K, lnaba M, Romani N, Ava H, Dezuchi M, Ikeh S, Muramatsu S, Steinman RM: Generation of large numb of dendritic cells from mouse bone marrow cultures supp mented with granulocyte/macrophage colony-stimulating tor. J Exp Med 176;1693-1702, 1992   DOI   ScienceOn
27 Porgador A, Yewdell JW, Deng Y, Bennink JR. Germain Localization, quantitation, and in situ detection of spec peptide-MHC class I complexes using monoclonal antibody. Immunity 6;715-726, 1997   DOI   ScienceOn
28 Jean FF, Daniel GK, Margareta L, Catherine S, Timothy Nina B, Marie L: Characterization of the MHC class I cro presentation pathway for cell-associated antigens by hum dendritic cells. Blood 102;4448-4455, 2003   DOI   ScienceOn
29 Saho U, Haruka F, Chie H, Masatoshi N, Mutsuhiko M: M class I-mediated exogenous antigen presentation by exosom secreted from immature and mature bone marrow deriv dendritic cells. Immunology Letters 89;125-131, 2003   DOI   ScienceOn
30 Zitvozel L, Reanault A, Lozier A, Wolfers J, Flament C, T za D, Ricciardi-Castagnoli P, Raposo G, Arnizorena S: Era cation of established murine tumors using a novel cell-f vaccine: dendritic cell-derived exosomes. Nat Med 4;594-600 1998   DOI   ScienceOn
31 Gong J, Avizan D, Chen D, Wu Z, Koido S, Kashiwaba Kufe D: Activation of antitumor cytotoxic T lymphocyte fusions of human dendritic cells and breast carcinoma ce Proc Natl Acad Sci 97;2715-2718, 2000
32 Palucka K, Bancherau J: Dendritic cells: a link between inn and adaptive immunity. J Clin Immunol 19;12-25, 1999   DOI   ScienceOn
33 Karttunen J, Sanderson S, Shastri N: Detection of rare an gen-presenting cells by the lacZ T-cell activation assay su gests an expression cloning strategy for T-cell antigens. Proc Natl Acad Sc 89;6020-6024, 1992
34 Yeh KY, McAdam AJ, Pulaski BA, Shastri N, Prelinzer Lord EM: IL-3 enhances both presentation of exogeno particulate antigen in association with class I major histoco patibility antigen and generation of primary tumor-spec cytolytic T lymphocytes. J Immunol 160;5773-5780, 1998   PUBMED