Browse > Article

B-1 Cells Differ from Conventional B (B-2) Cells: Difference in Proliferation  

Yeo, Seung Geun (Department of Otolaryngology, College of Medicine, Kyung Hee University)
Cho, Joong Saeng (Department of Otolaryngology, College of Medicine, Kyung Hee University)
Park, Dong Choon (Department of Obstetric and Gynecology, College of Medicine, Catholic University)
Rothstein, Thomas L. (Department of Medicine, School of Medicine and Immunobiology Unit, Evans Memorial Department of Clinical Research, Medical Center, Boston University)
Publication Information
IMMUNE NETWORK / v.4, no.3, 2004 , pp. 155-160 More about this Journal
Abstract
Background: B-1 cells differ from conventional B-2 cells both phenotypically and functionally. The aim of this study was to investigate the difference between peritoneal B-1 cells and splenic B-2 cells in proliferation. Methods: We obtained sorted B-1 cells from peritoneal fluid and B-2 cells from spleens of mice. During the culture of these cells, immunoglobulin secreted into the culture supernatants was evaluated by enzymelinked immunosorbent assay. Entering of S phase in response to LPS-stimuli was measured by proliferative assay. Results: Spontaneous Immunoglobulin M production occurred in peritoneal B-1 cells but not in splenic B-2 cells. LPS stimulated peritoneal B-1 cells secreted IgM at day 1, but splenic B-2 cells at day 2. In thymidine incorporation, peritoneal B-1 cells entered actively S phase after 24hours LPS-stimulation but splenic B-2 cells entered actively S phase after 48 hours. Conclusion: IgM secretion and S phase entering occurred early in peritoneal B-1 cells compared to splenic B-2 cells.
Keywords
Peritoneal B-1 cell; splenic B-2 cell; immunoglobulin.proliferation;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Hamilton AM, Lehuen A, Kearney JF: Immunofluorescence analysis of B-1 cell ontogeny in the mouse. Int Immunol 6;355-361, 1994   DOI   ScienceOn
2 Arita M, Kodama S, Suzuki M, Mogi G: Single cell analysis of adenoid CD5+B cells and their protective contributions to nasopharyngeal immunity. Laryngoscope 113;484-491, 2003   DOI   ScienceOn
3 Richard JU: Therapeutics targeting the innate immune system. Immunol 4: 512-520, 2004
4 Rothstein, TL.and Kolber DL: Anti-Ig Ab inhibits the phorbol ester-induced stimulation of peritoneal B cells. J Immunol 141;4089-4093, 1988
5 Morris DL, Rothstein TL: Analysis of B-1 cell activation: METHODS:A Companion to Methods in Enzymology 8;11-25, 1995   DOI   ScienceOn
6 박정수, 백수연, 신규태, 김도현, 남동호, 마경애, 윤정구, 장영주: 전신성 홍반성 낭창 환자의 혈액내 CD5+ B세포의 증가: 자가항체 농도와의 비연관성. 대한 면역학회지 22;23-30, 2000
7 이우길: 신생아 감염에서 CD5+ B세포의 증가. 대한면역학회지 19;399-406, 1997
8 Ferlin WG, Severinson E, Strom L, Heath AW, Coffman RL, Ferrick DA, Howard MC: CD40 signaling induces interleukin-4 independent IgE switching in vivo. Eur J Immunol 26;2911-2915, 1996   DOI   ScienceOn
9 Davey EJ, Thyberg J, Conrad D, Severinson E: Regulation of cell morphology in B lymphocytes by interleukin 4: Evidence for induced cytoskeletal changes. J Immunol 160;5366-5373, 1998
10 Piatelli MJ, Tanguay D, Rothstein TL, Chiles TC: Cell cycle control mechanisms in B-1 and B-2 lymphoid subsets. Immunol Res 27;31-52, 2003   DOI   ScienceOn
11 Tanguay DA, Colarusso TP, Doughty C, Pavlovic-Ewers S, Rothstein TL, Chiles TC: Cutting edge: differential signaling requirements for activation of assembled cyclin D3-cdk4 complexes in B-1 and B-2 lymphocyte subsets. J Immunol 166;4273-4277, 2001   DOI
12 노건웅, 강영순, 문인걸, 이우길: 알레르기 질환의 말포혈액림프구에서 CD5 mRNA 발현의 증가. 대한 면역학회지 19;347-353, 1997.
13 Sidman CL, Shultz RR, Hardy K, Hayakawa K, Herzenberg LA: Production of immunoglobulin isotypes by Ly-1+ B cells in viable motheaten and normal mice Science. 232;1423-1425, 1986   DOI   ScienceOn
14 Lalmanach-Girard AC, Chiles TC, Parker DC, Rothstein TL: T cell-dependent induction of NF-$\kappa$B in B cells. J Exp Med 177;1215-1219, 1993   DOI   ScienceOn
15 Morris DL, Rothstein TL: Decreased surface IgM receptor mediated activation of phospholipase Cr2 in B-1 lymphocytes. Int Immunol 6;1011-1016, 1994   DOI   ScienceOn
16 Calame KL, Lin KI, Tunyaplin C: Regulatory mechanisms that determine the development and function of plasma cells. Annu Rev Immunol 21;205-230, 2003   DOI   ScienceOn
17 노종열, 성원진, 성명훈, 이동욱, 박범정, 박석우, 김광현: 구개편도와 아데노이드에서 자연면역 림프구의 빈도와 분포. 한이인지 44;1073-1079, 2001.
18 Rothstein TL: Cutting Edge: Commentary:Two B-1 or Not To Be One. J of Immunology 168;4257-4261, 2002   DOI
19 Tanguay DA, Colarusso TP, Pavlovic S, irigoyen M, Howard RG, Bartek J, Chiles TC, Rothstein TL: Early induction of cyclin D2 expression in phorbol ester-responsive B-1 lymphocytes. J Exp Med. 189;1685-1690, 1999
20 Kawahara T, Ohdan H, Zhao G, Yang YG, Sykes M: Peritoneal cavity B cells are precursors of splenic IgM natural antibody-producing cells. J Immunol 171:5406-5414, 2003   DOI