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Registration and Intensity Compensation of Tilted Images of the Mitochondria Section Obtained from the Transmission Electron Microscopy  

Kim, Dong-Sik (Department of Electronics and Information Engineering, Hankuk University of Foreign Studies)
Publication Information
Journal of the Institute of Electronics Engineers of Korea SP / v.46, no.3, 2009 , pp. 1-9 More about this Journal
Abstract
Using the projected 2-dimensional tilted images obtained from the transmission electron microscopy, we can reconstruct the 3-dimensional structures of objects, such as cells. As a preprocessing procedure, the tilted images should be registered and compensated in terms of the spatial position and the intensity difference, respectively. In this paper, we employ the fiducial marker-based approach to perform a registration, and introduce a simple intensity compensation scheme. Based on the transmissivity image formation model, we propose an algorithm that can compensate the components of the illumination and transmissivity of each image according to the tilted angle. Numerical analysis using real images obtained from the transmission electron microscopy are shown in this paper to show the performance of the proposed algorithm.
Keywords
미토콘드리아;밝기보정;영상정렬;투과전자현미경;투사각 영상열;
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Times Cited By KSCI : 1  (Citation Analysis)
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