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http://dx.doi.org/10.5115/acb.2011.44.2.98

Gene expression profi ling of mouse aborted uterus induced by lipopolysac charide  

Moon, Jeong-Mi (Department of Anatomy, Research Institution of Medical Science, School of Medicine, Chonnam National University)
Lee, Song-Eun (Department of Anatomy, Research Institution of Medical Science, School of Medicine, Chonnam National University)
Min, Yong-Il (Department of Anatomy, Research Institution of Medical Science, School of Medicine, Chonnam National University)
Jung, Chae-Yong (Department of Anatomy, Research Institution of Medical Science, School of Medicine, Chonnam National University)
Ahn, Kyu-Youn (Department of Anatomy, Research Institution of Medical Science, School of Medicine, Chonnam National University)
Nam, Kwang-Il (Department of Anatomy, Research Institution of Medical Science, School of Medicine, Chonnam National University)
Publication Information
Anatomy and Cell Biology / v.44, no.2, 2011 , pp. 98-105 More about this Journal
Abstract
To identify genes that participate in the abortion process, normal pregnant uteri were compared to lipopolysaccharide (LPS)-induced abortion uteri. At day 6 of pregnancy, mice were treated with LPS at various time points to induce an abortion. Total RNAs were applied to a cDNA microarray to analyze genes with altered expression. At the early stage (2 hours) of LPS-induced abortion, upregulated genes were mainly composed of immune responsive genes, including Ccl4, Ccl2, Cxcl13, Gbp3, Gbp2, Mx2, H2-Eb1, Irf1 and Ifi203. Genes related to toll-like receptor signaling were also overexpressed. At late stages of abortion (12-24 hours), many genes were suppressed rather than activated, and these were mainly related to the extracellular matrix, cytoskeleton, and anti-apoptosis. Altered expression of several selected genes was confirmed by real time reverse transcription-polymerase chain reaction. Th e results demonstrated that many known genes were altered in the LPS-treated pregnant uterus, implying that the molecular mechanisms of the genes involved in LPS-induced abortion are complicated. Further analysis of this expression profile will help our understanding of the pathophysiological basis for abortion.
Keywords
Abortion; Lipopolysaccharide; Microarray; Mouse;
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