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http://dx.doi.org/10.5115/acb.2010.43.1.78

Chromatin organization and transcriptional activation of Hox genes  

Lee, Ji-Yeon (Department of Anatomy, Embryology Laboratory)
Min, Hye-Hyun (Department of Anatomy, Embryology Laboratory)
Wang, Xinnan (Department of Anatomy, Embryology Laboratory)
Khan, Abdul Aziz (Department of Anatomy, Embryology Laboratory)
Kim, Myoung-Hee (Department of Anatomy, Embryology Laboratory)
Publication Information
Anatomy and Cell Biology / v.43, no.1, 2010 , pp. 78-85 More about this Journal
Abstract
Spatially and temporally programmed expression of the Hox genes along the antero-posterior (A-P) axis is essential for correct pattern formation during embryonic development. An accumulating body of evidence indicates the pivotal role of spatial chromatin organization for the coordination of gene regulation. Recently, chromosome conformation capture (3C) technique has been developed and opened a new way to study chromosomal interactions in the nucleus. In this study, we describe 3C method we applied in F9 embryonic teratocarcinoma cells and demonstrate that the chromosomal interactions at Hox loci are successfully detected. Interestingly, at Hoxc loci, the abundance of intrachromosomal interactions with neighboring fragments was drastically decreased when the genes are expressed. These results indicate the possibility of the dynamic pattern of chromosomal interaction in association with the transcriptional regulation of Hox genes.
Keywords
Hox genes; chromosome conformation capture; spatial organization; chromosomal interaction; gene expression;
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