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Kinesin Superfamily KIF1Bα Protein Binds to the PDZ Domain of MALS-3  

Kim, Sang-Jin (인제대학교 의과대학 생화학교실)
Lee, Cheol-Hui (경상대학교)
Park, Hye-Yeong (인제대학교 의과대학 생화학교실)
Ye, Seong-Su (인제대학교 의과대학 생화학교실)
Jang, Won-Hui (인제대학교 의과대학 생화학교실)
Lee, Sang-Gyeong (인제대학교 의과대학 생화학교실)
Park, Yeong-Hong (인제대학교 의과대학 생화학교실)
Jeong, Yong-Uk (동국대학교 의과대학 해부학교실)
Seok, Dae-Hyeon (인제대학교 의과대학 생화학교실)
Publication Information
Anatomy and Cell Biology / v.39, no.5, 2006 , pp. 375-382 More about this Journal
Abstract
The Kinesin superfamily proteins (KIFs) make up a large superfamily of molecular motors that transport cargo such as vesicles, protein complexes, and organelles. KIF1Bα is a monomeric motor that conveys mitochondria and plays an important role in cellular function. Here, we used the yeast two-hybrid system to identify the proteins that interacts with KIF1Bα and found a specific interaction with the mammalian LIN-7 (MALS)- 3/vertebrate homology of LIN-7 (Veri) and synaptic scaffolding molecule (S-SCAM). MALS-3 protein bound to the tail region of KIF1Bα but not to other kinesin family members in the yeast two-hybrid assay. The “T-X-V” motif at the C-terminal end of KIF1Bα is essential for interaction with MALS-3. In addition, this protein showed specific interactions in the Glutathione S-transferase (GST) pull-down assay. An antibody to MALS-3 specifically coimmunoprecipitated KIF1Bα associated with MALS-3 from mouse brain extracts. These results suggest that MALS-3, as KIF1Bα receptor, is involved in the KIF1Bα-mediated transport.
Keywords
Kinesin; Molecular motors; Microtubule; Adaptor proteins; PDZ; MALS
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