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The Effect of Treatment with Imipramine and/or Tryptophan after Chronic Stress on the Serotonergic Immunoreactivity in Rat Raphe Nucleus  

Kim, Myoung-Soon (Faculty of Biotechnology, College of Oriental Medicine Woosuk University)
Lee, Chang-Hyun (Department of Anatomy, College of Oriental Medicine Woosuk University)
Publication Information
Anatomy and Cell Biology / v.35, no.5, 2002 , pp. 453-462 More about this Journal
Abstract
These experiments were performed to investigate the effect of saline, stress, imipramine, stress-imipramine and/or stress-tryptophan on serotonin immunoreactivity in raphe nucleus of the rats (200${\sim}$220 g, body weight). The animals were injected i.p. with imipramine (15 mg/kg) and tryptophan (15 mg/kg) after electric shocks for 20 days. The result by immunohistochemical methods were as follows; 1. Serotonin-immunoreactive neurons in the raphe nucleus of midbrain were significantly increased in stress treated group compared to saline treated group. 2. Serotonin-immunoreactive neurons in the raphe nucleus of midbrain were decreased in imipramine treated group compared all the other group. 3. Serotonin-immunoreactive neurons in the raphe nucleus of midbrain were significantly decreased in stress-imipramine treated group compared to stress alone treated group but were significantly increased in stress-imipramine treated group compared to imipramine treated group. 4. Serotonin-immunoreactive neurons in the raphe nucleus of midbrain were significantly increased in stress-tryptophan treated group compared to stress alone and saline treated group. These experiments indicated that serotonin immunoreactive neurons in raphe nucleus of midbrain were increased due to the activation of stress and decreased by suppresing activation of stress through imipramine treatment.
Keywords
Stress; Imipramine; Tryptophan; Midbrain; Raphe nucleus; Serotonin;
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1 Austin MC, O’Donnell SM : Regional distribution and cellular expression of tryptophan hydroxylase messenger RNA in postmortem human brainstem pineal gland, J Neurochem 72(5): 2065-2073, 1999
2 de Montigny C : Electroconvulsive treatments enhance responsiveness of forebrain neurons to serotonin, J pharmacol Exp Ther 228:230-234, 1984   PUBMED
3 Dilts RP, Boadle-Biber MC : Differential activation of the 5-hydroxytryptamine-containing neurons of the midbrain raphe of the rat in response to randomly presented inescapable sound, Neurosci Lett 199: 78-80, 1995
4 McEntee WJ, Crook TH : Serotonin, memory and the aging brain, Psychopharmacology 103: 143-149, 1991
5 Sette M, Raisman R, Briley M, Langer SZ : Localisation of tricyclic antidepressant binding sites on serotonin nerve terminals, J Neurochem 37(1): 40-42, 1981
6 Tagliamonte A, Tagliamonte P, Perez-Cruetz J, Stern S, Gessa GL : Effects of psychotropic tryptophan concentration in rat brain, J Pharmacol Exp Ther 177: 475-480, 1971
7 Chamas F, Serova L, Sabban EL : Tryptophan hydroxylase mRNA levels are elevated by repeated immobilization stress in rat raphe nuclei but not in pineal gland, Neurosci Lett 267: 157-160, 1999
8 de Montigny C, Aghajanian GK : Tricyclic antidepressants: long term treatment increases responsivity of rat forebrain neurons to serotonin, Science 202: 1303-1306, 1978
9 Sirvio J, Riekinen PJ, Jakala P : Experimental studies on the role serotonin in cognition, Prog Neurobiol 43: 363-379, 1994
10 Clement HW, Schafer F, Ruwe C, Gemsa D, Wesemann W : Sressinduced changes of extracellular 5-hydroxyindoleacetic acid concentrations followed in the nucleus raphe dorsalis and the frontal cortex of the rat, Brain Res 614: 117-124, 1993
11 Melia KR, Nestler EJ, Duman RS : Chronic imipramine treatment normalizes levels of tyrosine hydroxylase in the locus coeruleus of chronically stressed rats, Psychopharmacology 108(1-2): 23-26, 1992
12 Nestler EJ, McMahon A, Sabban EL, Tallman JF, Duman RS : Chronic antidepressant administration decreases the expression of tyrosine hydroxylase in the rat locus coeruleus, Proc Natl Acad Sci USA 87: 7522-7526, 1990
13 Adell A, Garcia-Marquez C, Armario A, Gelpi E : Chronic administration of chlomipramine prevents the increase in serotonin and noradrenaline induced by chronic stress, Psychopharmacology 99:22-26, 1989
14 Kim KS, Wessel TC, Stone DM, Carver CH, Joh TH, Park DH : Molecular cloning characterization of cDNA encoding tryptophan hydroxylase from rat central serotonergic neurons, Mol Brain Res 9: 277-283, 1991
15 Lovenberg W, Jequier E, Sjoerdsma A : Tryptophan hydroxylases measurement in pineal gland, brain stem, and carcinoid tumor, Science 155: 217-219, 1967
16 Thonen H : Induction of tyrosine hydroxylase in peripheral and central adrenergic neurons by cold exposure of rats, Nature 228: 861-862, 1970
17 Altman HJ, Normile HJ : What is the nature of the role of the serotonergic nervous system in learning and memory : prospects for development of an effective treatment strategy for senile dementia, Neurobiol Aging 9: 627-38, 1988
18 Friedman PA, Kappelman AG, Kaufmann S : Partial purification and characterisation of tryptophan hydroxylase from rabbit hindbrain, J Biol Chem 247: 4165-4173, 1972
19 Meltzer CC, Smith G, Dekosky ST, Pollock BG, Mathis CA, Moore RY, Kupfer DJ, Reynolds CF : Serotonin in aging, late-life depression, and Alzheimer’s desease: the emerging role of functional imaging, Neuropsychopharmacology 18(6): 407-427, 1998
20 Richard F, Faucon-Biguet N, Lavatut R, Rollet D, Mallet J, Buda M : Modulation of tyrosine hydroxylase gene expression in rat brain and adrenals by exposure to cold, J Neurosci Res 20: 32-37, 1988
21 Zigmond RE, Schon F, Iversen LL : Increased tyrosine hydroxlase activity in the locus coeruleus of rat brain stem after reserpine treatment and cold stress, Brain Res 70: 547-555, 1974
22 Virendra BS, Karl CC, Tom-Hao P, boadle-Biber MC : Increases in the actiyity of tryptophan hydroxlase from rat cortex and midbrain in response to acute or repeated sound stress are blocked by adrenalectomy and restored by dexamethasone treatment, Brain Res 516 : 66-76, 1990
23 Amat J, Matus-Amat P, Watkins LR, Majer SF : Escapable and inescapable stress diffentially and selectively alter extracellular levels of 5HT in the ventral hippocampus and dorsal periaqueductal gray of the rat, Brain Res 797: 12-22, 1998
24 Azmita EC, Jr., McEwen BS : Adernalcortical influence on rat brain tryptophan hydroxlase activity, Brain Res 78: 291-302, 1974
25 Knott PJ, Joseph MH, Curzon G : Effects of food deprivation and immobilization on tryptophan and other amino acids in rat brain, J Neurochem 20: 249-251, 1973
26 Culman J, Kiss A, Kvetnansky R : Serotonin and Tryptophan hydroxlase in isolated hypothalamic and brain stem nuclie of rats exposed to acute and repeated immobilization stess, Exp Clin Endocrinol 83: 28-36, 1984
27 Handley SL : 5-hydroxytryptamine pathways in anxiety and its treatment, Pharmacol Ther 66: 103-148, 1995
28 김명순, 이창현 : Tryptophan과 imipramine처리가 흰쥐의 중뇌 솔기핵의 serotonin 면역 반응 신경세포체에 미치는 영향, 대한해부학회지 35(1): 83-90, 2002
29 Adell A, Garcia-Marquez C, Armario A, Gelpi E : Chronic stress increases serotonin and noradrenaline in rat brain and sensitizes their responses to a further acute stress, J Neurochem 50: 1678-1681, 1988
30 Fernstrom JD, Wurtman RJ : Brain serotonin content: Physiological dependence on plasma tryptophan levels, Science 173: 149-152, 1971
31 Gold PW, Goodwin FK, Chrousos GP : Clinical and biochemical manifestations of depression : relation to the neurobiology of stress, N Engl J Med 319: 348-353, 1988
32 Blier P, de Montigny C : Current advances and trends in the treatment of depression, Trends Pharmacol Sci 15: 220-226, 1994
33 Van de Kar LD, Lorens SA : Differential serotonergic innervation of individual hypothalamic nuclei and other forebrain regions by the dorsal and median midbrain raphe nuclei, Brain Res 162: 45-54, 1979
34 Willner P : The validity of animal models of depression, Psychopharmacology 83: 1-6, 1984
35 Heninger GR, Charney DS : Mechanisms of action of antidepressant treatments: implications for the etiology and treatment of depression disorders, In: Psychopharmacology: the third generation of progress: 535-544, 1987