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http://dx.doi.org/10.4491/KSEE.2017.39.5.265

Application of Enzymatic Activity and Arsenic Respiratory Gene Quantification to Evaluate the Ecological Functional State of Stabilized Soils Nearby Closed Mines  

Park, Jae Eun (Department of Environmental Science and Engineering, Ewha Womans University)
Lee, Byung-Tae (Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology)
Lee, Sang Woo (Department of Geology and Research Institute of Natural Science, Gyeongsang National University)
Kim, Soon-Oh (Department of Geology and Research Institute of Natural Science, Gyeongsang National University)
Son, Ahjeong (Department of Environmental Science and Engineering, Ewha Womans University)
Publication Information
Journal of Korean Society of Environmental Engineers / v.39, no.5, 2017 , pp. 265-276 More about this Journal
Abstract
Heavy metals leaching from closed mines have been causing severe environmental problems in nearby soil ecosystems. Mine reclamation in Korea has been recently implemented based on the heavy metal immobilization (a.k.a., stabilization). Since the immobilization temporarily fixes the heavy metals to the soil matrix, the potential risk of heavy metal leaching still exists. Therefore the appropriate monitoring and the related policies are required to safeguard the soils, where all the cultivations occur. The current monitoring methods are based on either heavy metal concentration or simple toxicity test. Those methods, however, are fragmented and hence it is difficult to evaluate the site in an integrated manner. In this study, as the integrated approach, ecological functional state evaluation with a multivariate statistical tool was employed targeting physiochemical soil properties, heavy metal concentrations, microbial enzymatic activity, and arsenic respiratory reductase gene quantity. Total 60 soil samples obtained from three mines (Pungjeong, Jeomdong, Seosung) were analyzed. As a result, the stabilized layer soil and lower layer soil have shown the similar pattern in Pungjeong mine. In contrast, Jeomdong and Seosung mine have shown the similarity between the stabilized layer soil and the cover layer soil, indicating the possible contamination of the cover layer soil.
Keywords
Heavy Metal Stabilization; Ecological Functional State; Microbial Enzymatic Activity; Arsenic Respiratory Reductase Gene; Multivariate Statistical Analysis;
Citations & Related Records
Times Cited By KSCI : 8  (Citation Analysis)
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1 Smith, J. L. and Doran, J. W., "Chap 10. Measurement and use of pH and electrical conductivity for soil quality analysis," Methods for assessing soil quality, 169-185(1996). SSSA Special Publication, Soil Science Society of America, Madison, WI, USA
2 Doran, J. W., Coleman, D. C., Bezdicek, D. F. and Stewart, B. A. and Timothy, B. P., (ed) Defining soil quality for a sustainable environment, "Chap 1. Defining and assessing soil quality," pp. 1-21(1994).
3 Schoenholtz, S. H., Van Miegroet, H. and Burger, J. A., "A review of chemical and physical properties as indicators of forest soil quality: challenges and opportunities," Forest Ecol. and Manage., 138(1), 335-356(2000).   DOI
4 Jordan, D., Kremer, J., Bergfield, W. A., Kim, K. Y. and Cacnio, V. N., "Evaluation of microbial methods as potential indicators of soil quality in historical agricultural fields," Biol. and Fertility of Soils, 19(4), 297-302(1995).   DOI
5 Kim, M., Jung, J.-W. and Nam, K., "The Toxicity Assessment of Explosives Contaminated Soil using Soil Microbial Activity Tests," J. Soil and Groundwater Environ., 20(6), 37-45(2015).   DOI
6 Ki, D., Park, J, Lee, J. and Rho, P., "A weak correlation of field-detemined soil microbial diversity with quantitative ecological map information and its methodological implication in estimation soil ecological quality," J. Korean Civil Eng., 27(6), 703-710(2007).
7 Filip, Z. "International approach to assessing soil quality by ecologically-related biological parameters," Agric., Ecosystems & Environ., 88(2), 169-174(2002).   DOI
8 Son, J.-H., Roh, H., Lee, S.-Y., Kim, S.-K., Kim, G.-H., Park, J.-K., Yang, J.-K. and Chang, Y.-Y., "Stabilization of heavy metal contaminated paddy soils near abandoned mine with steel slag and CaO," J. Soil and Groundwater Environ., 14(6), 78-86(2009).
9 Koh, I.-H., Lee, S.-H., Lee, W.-S. and Chang, Y.-Y., "Assessment on the transition of arsenic and heavy metal from soil to plant according to stabilization process using limestone and steelmaking slag," J. Soil and Groundwater Environ., 18(7), 63-72(2013).   DOI
10 Oh, S.-J., Kim, S.-C., Yoon, H.-S., Kim, H.-N., Kim, T.-H., Yeon, K.-H., Lee, J.-S., Hong, S.-J. and Yang, J.-E., "Evaluating heavy metal stabilization efficiency of chemical amendment in agricultural field: field experiment," Korean J. Soil Sci. and Fertilizer, 44(6), 1052-1062(2011).   DOI
11 Doran, J. W. and Zeiss, M. R, "Soil health and sustainability: managing the biotic component of soil quality," Appl. Soil Ecol., 15(1), 3-11(2000).   DOI
12 Knoepp, J. D., Coleman, D. C., Crossley Jr., D. A. and Clark, J. S., "Biological indices of soil quality: an ecosystem case study of their use," Forest Ecol. and Manage., 138(1), 357-368(2000).   DOI
13 Dorn, P. B., Vipond, T. E., Salanitro, J. P. and Wisniewski, H. L., "Assessment of the acute toxicity of crude oils in soils using earthworms, $microtox^{(R)}$, and plants," Chemosphere, 37(5), 845-860(1998).   DOI
14 Jones, D. L., Rousk., J., Edwards-Jones, G., DeLuca, T. H. and Murphy, D. V., "Biochar-mediated changes in soil quality and plant growth in a three year field trial," Soil Biol. and Biochem., 45(2), 113-124(2012).   DOI
15 Stohlgren, T. J., Schell, L. D. and Heuvel, B. V., "How grazing and soil quality affect native and exotic plant diversity in Rocky Mountain grasslands," Ecol. Appl., 9(1), 45-64(1999).   DOI
16 Jung, M. C., Ahn, J. S. and Chon, H. T., "Environmental contamination and sequential extraction of trace elements from mine wastes around various metalliferous mines in Korea," Geosyst. Eng., 4, 50-60(2001).   DOI
17 ASTM International, "Standard test methods for loss of ignition(LOI) of solid combustion residues," ASTM D7348.
18 Tyler, G., "Heavy metal pollution and soil enzymatic activity," Plant and Soil, 41(2), 303-311(1974).   DOI
19 Mine Reclamation Corporation, "The second stage of mine reclamation plan (year of 2012-2016)," Ministry of Knowledge Economy(2011).
20 Jung, M. C., Jung, M. Y. and Choi, Y. W., "Environmental Assessment of Heavy Metals Around Abandoned Metalliferous Mine in Korea," Economic and Environ. Geol., 37(1), 21-33(2004).
21 Tica, D., Udovic, M. and Lestan, D., "Long-term efficiency of soil stabilization with apatite and Slovakite: The impact of two earthworm species (Lumbricus terrestris and Dendrobaena veneta) on lead bioaccessibility and soil functioning," Chemosphere, 91(1), 1-6(2013).   DOI
22 Kim, J. H., Chung, D. Y., Oh, S. J., Kim, R. Y., Yang, J. E., Park, G. I., Lee, J. S. and Kim, S. C., "Determining soil quality of heavy metal contaminated agricultural field in Korea," Korean J. Soil Sci. and Fertilizer, 45(6), 1237-1241(2012).   DOI
23 Burns, R. G., "Enzyme activity in soil: location and a possible role in microbial ecology," Soil Biol. and Biochem., 14(5), 423-427(1982).   DOI
24 Knight, T. R. and Dick, R. P., "Differentiating microbial and stabilized ${\beta}$-glucosidase activity relative to soil quality," Soil Biol. and Biochem., 36(12), 2089-2096(2004).   DOI
25 Afkar, E., Lisak, J., Saltikov, C., Basu, P., Oremland, R. S. and Stolz, J. F., "The respiratory arsenate reductase from Bacillus selenitireducens strain MLS10," FEMS Microbiol. Lett., 226(1). 107-112(2003).   DOI
26 Oh, S. J., Kim, S. C., Kim, R. Y., Ok, Y. S., Oh, S. M., Lee, J. S. and Yang, J. E., "Change of bioavailability in heavy metal contaminated soil by chemical amendment," Korean J. Soil Sci. Fertilizer, 45(6), 973-982(2012).   DOI
27 Park, Y. H. and Seo, K. W., "Policy Suggestions for Soil Contamination Prevention and Management of Inactive or Abandoned Metal Mines," J. Korean Soc. Soil and Groundwater Environ., 11(3), 1-11(2006).
28 Kim, S. O., Moon, S. H. and Kim, K. W., "A study on the removal of heavy metals from soils using electrokinetic soil processing and ion exchange membrane," Economic and Environ. Geol., 32(1), 43-51(1991).
29 Kim, J.-Y., Davis, A. P. and Kim, K.-W. "Stabilization of available arsenic in highly contaminated mine tailings using iron," Environ. Sci. Technol., 37(1), 189-195(2003).   DOI
30 Ministry of Environment, "Soil pollution process test standard," webbook.me.go.kr/DLi-File/NIER/09/018/5509908.pdf (2009).
31 Lee, Y.-H. and Ha, S.-K., "Impacts of chemical properties on microbial population from upland soils in Gyeongnam Province," Korean J. Soil Sci. and Fertilizer, 44(2), 242-247(2011).   DOI
32 Malasarn, D., Saltikov, C. W., Campbell, K. M., Santini, J. M., Hering, J. G. and Newman, D. K., "arrA is a reliable marker for As(V) respiration," Science, 306(5695), 455-455(2004).   DOI
33 Kulp, T. R., Hoeft, S. E., Miller, L. G., Saltikov, C., Murphy, J. N., Han, S., Lanoil, B. and Oremland, R. S., "Dissimilatory arsenate and sulfate reduction in sediments of two hypersaline, arsenic-rich soda lakes: Mono and Searles Lakes, California," Appl. and Environ. Microbiol., 72(10), 6514-6526(2006).   DOI
34 Paez-Espino, D., Tamames, J., de Lorenzo, V. and Canovas, D. "Microbial responses to environmental arsenic," Biometals, 22(1), 117-130(2009).   DOI