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http://dx.doi.org/10.4491/KSEE.2016.38.4.177

Effect of ZnO and TiO2 Nanopaticles (NPs) on Microorganisms Growth in Activated Sludge  

Ha, Min Jeong (Graduate School of Water Resources, Sungkyunkwan University)
Lee, Yeo Eun (Graduate School of Water Resources, Sungkyunkwan University)
Jang, Am (Graduate School of Water Resources, Sungkyunkwan University)
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Abstract
Sewage treatment using microorganisms is affected by multiple factors such as microbial properties, characteristics of sewage and operating conditions, and nanoparticles inflow may cause negative effects on sewage treatment system especially on the system stability and efficiency. It was studied to assess the toxic effects of nanoparticles on microorganism growth. The activated sludge in the sewage treatment plant of university was cultured in the optimized medium for each strain. Bacillus (gram-positive), Pseudomonas and E.coli (gram-negative) in the activated sludge were selected as target microorganisms, and ZnO and $TiO_2$ were chosen as nanoparticles. For same concentration of nanoparticles, average growth inhibition rate of Bacillus was 60% or more, while that of Pseudomonas was less than 10%. The toxicity of nanoparticles was shown to be higher for gram-positive bacteria than gram-negative bacteria because of their differences on structure of cell wall, components of cell wall protein, physiology of cells and metabolism. ZnO affected 3 times more negative on the growth of microorganisms as compared to $TiO_2$. It was assumed that, therefore, toxicity of ZnO was found to be greater than $TiO_2$.
Keywords
Activated Sludge; Growth Rate of Microorganisms; ZnO Nanoparticles; $TiO_2$ Nanoparticles;
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