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http://dx.doi.org/10.4491/KSEE.2015.37.6.363

Production of Polyhydroxyalkanoates (PHAs) from Sequencing Batch Reactor Using Synthetic Wastewater  

Son, Jae-hyup (Department of Civil and Environmental Engineering, University of Ulsan)
Cha, Sang-Hyeop (Department of Civil and Environmental Engineering, University of Ulsan)
Park, Jun-mo (Department of Civil and Environmental Engineering, University of Ulsan)
Park, Hung-suck (Department of Civil and Environmental Engineering, University of Ulsan)
Publication Information
Journal of Korean Society of Environmental Engineers / v.37, no.6, 2015 , pp. 363-370 More about this Journal
Abstract
The production of polyhydroxyalkanoates (PHAs) using Sequencing Batch Reactor (SBR) was investigated. The experiments were performed in two fabricated SBRs (4 L) of different oxidation state. Synthetic wastewater was used as substrate, using C/N/P ratio of 42:10:1. SBR 1 and SBR 2 were operated in aerobic dynamic feeding (ADF) and anaerobic/oxic dynamic feeding (AODF) condition, respectively. ADF provide feast and famine in aerobic condition, while AODF in anaerobic/oxic condition. PHAs production was found high in AODF than AOF. Maximum PHAs content of 40.0% (w/w)of biomass were produced in AODF mode. Produced PHAs structural and thermal property were good.
Keywords
Sludge; Bioplastic; Polyhydroxyalkanoates (PHAs) Production; Biodegradable Polymer; Biosynthesis;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Satoh, H., Mino, T. and Matsuo, T., "Anaerobic uptake of glutamate and aspartate by enhanced biological phosphorus removal activated sludge," Water Sci. Technol.; Micro. Activ. Sludge and Biofilm Proc. II Proc. 2nd Int. Confe. Micro. Activ. Sludge and Biofilm Proc., 37(4), 579-582(1998).
2 Bengtsson, S., Werker, A., Christensson, M. and Welander, T., "Production of polyhydroxyalkanoates by activated sludge treating a paper mill wastewater," Bioresour. Technol., 99(3), 509-516(2008).   DOI   ScienceOn
3 Mengmeng, C., Hong, C., Qingliang, Z., Shirley, S. N. and Jie, R., "Optimal production of polyhydroxyalkanoates (PHA) in activated sludge fed by volatile fatty acids (VFAs) generated from alkaline excess sludge fermentation," Bioresour. Technol., 100(3), 1399-1405(2009).   DOI   ScienceOn
4 Park, T. J. and Lee, S. Y., "A Research and Application of Polyhydroxyalkanoates in Biosensor Chip," J. KSBB, 22(6), 371-377(2007).
5 Park D. H. and Kim, B. S., "Characteristics of Polyhydroxyalkanoates Synthesis by Ralstonia eutropha from Vegetable Oils," J. KSBB, 25(19), 239-243(2010).
6 Wen, Q., Chen, Z., Wang, C. and Ren, N., "Bulking sludge for PHA production: Energy saving and comparative storage capacity with well-settled sludge," J. Environ. Sci., 24(10), 1744-1752(2012).   DOI
7 Salmiati, Z. U., Salim, M. R. and Olsson, G., "Recovery of polyhydroxyalkanoates (PHAs) from mixed microbial cultures by simple digestion and saponification," In The 3rd IWA-Aspire, Conference and Exhibition. Working for Asia-Pacific Water Sustainability,(2009).
8 Shah, K., "FTIR analysis of polyhydroxyalkanoates by a locally isolated novel bacillus sp. AS 3-2 from soil of kadi region, north gujarat, india," J. Biochem. Technol., 3(4), 380-383(2012).
9 Ministry of Environment (South Korea), (2013) Statistics of Sewerage, pp. 1458-1459(2014).
10 Ministry of Environment (South Korea), Sewerage Division, "Fundamental plan for energy independence," pp. 4-10(2010).
11 Kim, M. J., "Bio material utilization technology (Research Focusing on bioplastic)," KRIBB, BT report, pp. 3-5(2012).
12 Small and Medium Business Administration, "Green production: Eco-friendly production," 2013 Technology Road Map for SME, pp. 43-45(2013).
13 Yoo, Y. S., "About Bio-plastic," Korea Bio Mater. Packag. Assoc., The Monthly Packag. World, 217(2), 45-56(2011).
14 Jegal, J. G., "State and Prospects of Bioplastics," J. Appl. Chem. Eng., 15(4), 21-25(2012).
15 Chua, A. S., Takabatake, H., Satoh, H. and Mino, T., "Production of polyhydroxyalkanoates (PHA) by activated sludge treating municipal wastewater: Effect of pH, sludge retention time (SRT), and acetate concentration in influent," Water Res., 37(15), 3602-3611(2003).   DOI   ScienceOn
16 De Rijk, T. C., Van de Meer, P., Eggink, G. and Weusthuis, R. A., "Methods for analysis of poly(3-hydroxyalkanoate) (PHA) composition," Biopolymers Online, Wiley-VCH Verlag GmbH & Co., (2005).
17 Koller, M. and Niebelschutz, H., "Gerhart Braunegg, Strategies for recovery and purification of poly[(R)-3-hydroxyalkanoates] (PHA) biopolyesters from surrounding biomass," Eng. Life Sci., 13(6), 549-562(2013).   DOI   ScienceOn
18 Riis V. and Mai W., "Gas chromatographic determination of poly-${\beta}$-hydroxybutyric acid in microbial biomass after hydrochloric acid propanolysis," J. Chromatogr. A, 445, 285-289(1988).   DOI   ScienceOn
19 Yu, J., "Production of PHA from starchy wastewater via organic acids," J. Biotechnol., 86(2), 105-112(2001).   DOI   ScienceOn
20 Cho, K.-S., Ryu, H.-W. and Park, C.-H., "Production of Polyhydroxyalkanoates from Swine Wastewater," J. Korean Soc. Environ. Eng., 18(10), 1259-1270(1996).
21 Salehizadeh, H. and Van Loosdrecht, M. C. M., "Production of polyhydroxyalkanoates by mixed culture: Recent trends and biotechnological importance," Biotechnol. Adv., 22(3), 261-279(2004).   DOI   ScienceOn
22 Shamala, T. R., Chandrashekar, A., Vijayendra, S. V. N. and Kshama, L., "Identification of polyhydroxyalkanoate (PHA)-producing bacillus spp. using the polymerase chain reaction (PCR)," J. Appl. Microbiol., 94(3), 369-374(2003).   DOI   ScienceOn
23 Otari, S. and Ghosh, J., "Production and characterization of the polymer polyhydroxy butyrate-copolyhydroxy valerate by bacillus megaterium NCIM 2475," Curr. Res. J. Biol. Sci., 1(2), 23-26(2009).
24 Rawte, T. S. M., "Characterization of polyhydroxy alkanoates-biodegradable plastics from marine bacteria," Curr. Sci., 83(5), 562-564(2002).
25 Lee, W. Y., Yoo, D. H., Kim, J. Y. and Choi, U., "Factors affecting the production of PHA (polyhydroxyalkanoates) using SBR," In proceeding of the Spring Annual Conference of KSWW, J. Korean Soc. Water, 2001(4), 269-272(2001).
26 Wang, D., Chen, Y., Zheng, X., Li, X. and Feng, L., "Short-Chain Fatty Acid Production from Different Biological Phosphorus Removal Sludges: The Influences of PHA and Gram-Staining Bacteria," J. Environ. Sci. Technol., 47(6), 2688-2695(2013).   DOI   ScienceOn