Browse > Article
http://dx.doi.org/10.4491/KSEE.2014.36.10.672

Variations of Hydrogen Production and Microbial Community with Different Nitrogen Concentration During Food Waste Fermentation  

Lee, Pul-Eip (Department of Environmental Engineering, Seoul National University of Science & Technology)
Lee, Tae-Jin (Department of Environmental Engineering, Seoul National University of Science & Technology)
Publication Information
Journal of Korean Society of Environmental Engineers / v.36, no.10, 2014 , pp. 672-678 More about this Journal
Abstract
In this study, variations of fermentative hydrogen production and microbial community were investigated with different nitrogen concentration of food waste. Optimum hydrogen production rate was acquired at 200 mg/L nitrogen concentration of the food waste. Which was eqivalent to 83.43 mL/g dry biomass/hr. However, bio-hydrogen production was inhibitedly reduced at over 600 mg/L of nitrogen concentration whereas proportional relation between hydrogen production and B/A ratio were not observed. Most dominant specie of the microbial community analyzed was Clostridium sp. throughout PCR-DGGE analysis of 16S rDNA. It revealed that most contributing microorganism producing hydrogen were Enterococcus faecium partial, Klebsiella pneumoniae strain ND6, Enterobacter sp. NCCP-231, and Clostridium algidicarnis strain E107 in this experiment.
Keywords
Food Waste; Anaerobic Fermentation; Hydrogen Production; Nitrogen Concentration; PCR-DGGE;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Logan, B. E., OH, S. E., Kim, I. S. and Ginkel, S. V., "Biological hydrogen production measured in batch anaerobic respirometers," Environ. Sci. Technol., 36(11), 2530-2535(2002).   DOI   ScienceOn
2 Okamoto, M., Miyahara, T. Mizuno, O. and Noike, T. Biological hydrogen potential of materials characteristic of the organic fraction of municipal solid wastes," Water Sci. Technol., 41(3), 25-32(2000).
3 Kang, J.-H., Kim, D. K. and Lee, T.-J., "Hydrogen production and microbial diversity in sewage sludge fermentation preceded by heat and alkaline treatment," Bioresour. Technol., 109, 239-243(2012).   DOI   ScienceOn
4 Ozkan, L., Tuba, H. E., Goksel, N. D., "Effects of pretreatment methods on solubilization of beet-pulp and bio-hydrogen production yield," Int. J. Hydro. Energy, 36(1), 382-389(2011).   DOI   ScienceOn
5 Jun, Y.-S., Joe, Y.-A. and Lee., T.-J., "Change of Microbial Community and Fermentative Production of Hydrogen from Tofu Wastewater," J. Kor. Soc. Environ. Eng., 31(2), 139-146(2009).   과학기술학회마을
6 Chen, X., Sun, Y., Xiu, Z., Li, X. and Zhang, D., "Stoichiometric analysis of biological hydrogen production by fermentative bacteria," Int. J. Hydro. Energy, 31(4), 539-549(2006).   DOI   ScienceOn
7 Ueno, Y., Haruta, S., Ishii, M. and Igarashi, Y., "Characterization of a microorganism isolated from the effluent of hydrogen fermentation by microflora," J. Biosci. Bioeng., 92(4), 397-400(2001).   DOI   ScienceOn
8 Hiraishi, A., "Respiratory quinone profiles as tool for identifying different bacterial populations in activated sludge.," J. Gen. Appl. Microbiol., 34(1), 39-56(1988).   DOI
9 Hollibaugh, J. T., Bano, N. and Ducklow, H., "Widespread distribution in polar oceans of a 16S rRNA gene sequence with affinity to Nitrosospira-like ammonia-oxidizing bacteria," Appl. Environ. Microbiol., 68(3), 1478-1484(2002).   DOI   ScienceOn
10 Amann, R, I., Ludwig, W. and Schleifer, K.-H., "Phylogenetic identification and insitu detection of individual microbial cells without cultivation," Microbiol. Rev., 59(1), 143-169(1995).
11 Kimura, M., "A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences," J. Mol. Evol., 16(2), 111-120(1980).   DOI   ScienceOn
12 Saitou, N. and Nei, M., "The neighbour-joining method: a new method for constructing phylogenetic trees," Mol. Biol. Evol., 4(4), 406-425(1987).
13 Lee., S. M., Park., J. L., Ahn., J. S., "The Study on the Alcohol Extraction from Organic Wastes by Anaerobic Digestion," J. Kor. Soc. Waste Manage., 3(2), 49-64(1986).
14 Zhang, S., Lee, Y.-H., Km, T.-H. and Hwang, S.-J., "Effects of NaCl Concentrations on Hydrogen Production and Microbial Community by Dark-fermentation," J. Kor. Soc. Waste Manage., 39(1), 46-51(2013).
15 Scott, D. S., "Hydrogen-the case for inevitability," Int. J. Hydro. Energy, 29(3), 225-227(2004).
16 Korea Ministry of environment Homepage, http://www.me.go.kr(2005).
17 Chen., Y., Cheng, J. J., Creamer, K. S., "Inhibition of anaerobic digestion process: a review," Bioresour. Technol., 99(10), 4044-4064(2008).   DOI   ScienceOn
18 Kim, D.-H., Kim, S.-H., Shin, H.-S., "Sodium inhibition of fermentative hydrogen production," Inter. J. Hydro. Energy, 34(8), 3295-3304(2009).   DOI   ScienceOn
19 Debabrata, D. and Veziroglu, T. N., "Hydrogen production by biological processes: a survey of literature," Int. J. Hydro. Energy, 26(1), 13-28(2001).   DOI   ScienceOn
20 Rifkin, J., "The hydrogen economy: the worldwide energy web and the redistribution of the power on earth," Penguin Putnam, New Work, US, pp. 15-17(2002).
21 Mizuno, O., Ohara, T., Shinya, M. and Noike, T., "Characteristics of hydrogen production from bean curd manufacturing waste by anaerobic microflora," Water Sci. Technol., 42(3), 345-350(2000).
22 James, T. H., Nasreen, B. and Hugh, W., "Widespread distribution in polar oceans of a 16S rRNA gene sequence with affinity to Nitrosospira-like ammonia-oxidizing bacteria," Appl. Environ. Microbiol., 68(3), 1478-1484(2002).   DOI   ScienceOn
23 Mannix, S. P., Shin, H., Masaru, H., Rumiko, S., Chie, Y., Koichiro, H., Masaharu, I. and Yasuo, I., "Denaturing gradient gel electrophoresis analyses of microbial community from field-scale composter," J. Biosci. Bioeng., 91(2), 159-165(2001).   DOI   ScienceOn
24 Herbert, H. P. F. and Hong, L., "Effect of pH on hydrogen production from glucose by a mixed culture," Bioresour. Technol., 82(1), 87-93(2002).   DOI   ScienceOn
25 Chen., C. C. and Lin, C. Y., "Using sucrose as a substrate in an anaerobic hydrogen-producing reactor," Adv. Environ. Res., 7(3), 695-699(2003).   DOI   ScienceOn
26 Auch, A. F., Henz, S. R., Holland, B. R. and Goker, M., "Genome BLAST distance phylogenies inferred from whole plastid and whole mitochondrion genome sequences," Bio-Med Central Bioinformatics, 7(350), 1-16(2006).
27 Sudhir, K., Masatoshi, N., Joel, D. and Koichiro, T., "MEGA: A biologist-centric software for evolutionary analysis of DNA and protein sequences," Brierings Bioinformatics, 9(4), 299-306(2008).   DOI   ScienceOn
28 Felgenstein, J., "Confidence limits on phylogenetics: an approach using the bootstrap," Evolution, 39, 783-791(1985).   DOI   ScienceOn
29 Wesley, W. and Jr., Eckenfelder, Industrial Water Pollution Control, 3rd ed,, Mc Graw-Hill, New york, pp. 422(2000).