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http://dx.doi.org/10.4491/KSEE.2013.35.7.503

Analysis of Microbial Communities in Animal Carcass Disposal Soils  

Park, Jeong-Ann (Environmental Functional Materials and Biocolloids Laboratory, Seoul National University)
Choi, Nag-Choul (Environmental Functional Materials and Biocolloids Laboratory, Seoul National University)
Kim, Song-Bae (Department of Rural Systems Engineering, Seoul National University)
Publication Information
Journal of Korean Society of Environmental Engineers / v.35, no.7, 2013 , pp. 503-508 More about this Journal
Abstract
The aim of this study was to investigate the microbial communities in animal carcass disposal soils to examine the possible threat of pathogens from leachate. DNA extraction was performed for the soils in three carcass disposal sites located in Gyeonggi-do, Korea, and then 16S rRNA pyrosequencing was conducted to identify the microbial communities. Results indicate that, according to phylum classification, Proteobacteria (100%) was identified in soil A, Actinobacteria (66.4%) > Proteobacteria (31.1%) > Bacteriodetes (2.1%) > Acidobacteria (0.3%) in soil B, and Actinobacteria (63.1%) > Proteobacteria (36.9%) in soil C. According to genus classification, Pseudomonas was dominant in soil A (98%), Arthrobacter in soil B (68%) and C (61%). There were no detections of pathogens such as Salmonella, Campylobacter and Clostridium perfringens. However, high concentration of Ralstonia pickettii causing bacteremia was observed. Although carcass disposal soils examined in this study were not highly contaminated with pathogens, further monitoring is still needed to examine the potential threat of pathogens in leachate derived from carcass disposal sites.
Keywords
Animal Carcass Disposal Soils; Bacteria; Microbial Community; 16S rRNA Pyrosequencing; Pathogens;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
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