Browse > Article

Changes of Microbial Community Structure According to a Changes of Season and Influent Characteristics in Biological Wastewater Treatment  

Son, Hyeng-Sik (Department of Microbiology, Pusan National University)
Son, Hee-Jong (Busan Water Authority)
Kim, Mi-A (Department of Microbiology, Pusan National University)
Ryu, Eun-Yeon (Korea Bio-IT Foundry Center, Pusan National University)
Lee, Geon (Sejung Biotech Co. Ltd.)
Lee, Sang-Joon (Department of Microbiology, Pusan National University)
Publication Information
Abstract
The bacterial community structure in biological reactor in wastewater treatment system was investigated by denaturing gradient gel electrophoresis (DGGE) and fluorescent in situ hybridization (FISH). Samples were collected at different three points in wastewater treatment system. Through treatment processes, BOD (biochemical oxygen demand) and COD (chemical oxygen demand) of was removal efficiency was 83.1~98.6%, 67.2~85.2% respectively. Microbial community of aerobic tank and oxic tank were similar but anoxic tank was different (RRP group was increased about tripple) by DGGE and FISH in sludge (2007 October and 2008 January). Samples in 2007 October and 2008 January were dominant ${\alpha}$-Proteobacteria and CF group respectively. Sludge in 2008 April were different comparing former results dominant others as 65~80%. Others group was dominant. Eubacteria by FISH with the probe EUB338 was about $1.7{\sim}7.6{\times}10^9\;cells/mL$. It could be successfully observed bacterial community in biological wastewater system.
Keywords
Microbial Community; DGGE; FISH; Biological Wastewater Treatment;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 김동진, 한동우, 이수철, 박병곤, 권일, 성창근, 박완철, "생물막 반응기에서의 폐수 처리 및 fluorescence in situ hybridization," 한국생물공학회지, 17(1), 80-87(2002).   과학기술학회마을
2 Nielsen, P. H., Andreasen, K., Wagner, M., Blackall, L. L., Lemmer, H. and Seviour, R. J., "Variability of type 021N in activated sludge as determined by in situ substrate uptake pattern and in situ hybridization with fluorescent rRNA targeted probes," Water Sci. Technol., 37, 423-440(1998).   DOI   ScienceOn
3 Ohashi, A., Viraj de Silva, D. G., Mobarry, B., Manem, J. A., Stahl, D. A. and Rittmann, B. E., "Influence of substrate C/N ratio on the structure of multi-species biofilms consisting of nitrifiers and heterotrophs," Water Sci. Technol., 32(8), 75-84(1995).   DOI   ScienceOn
4 Dong, X. and Reddy, G. B., "Soil bacterial communities in constructed wetlands treated with swine wastewater using PCR-DGGE technique," Bioresour. Technol., 101, 1175-1182 (2010).   DOI   ScienceOn
5 Kabdasli, I., Tunay, O. and Orhon, D., "The treatability of chromium tannery wastes," Water Sci. Technol., 28, 97-105 (1993).
6 Glockner, F. O., Fuchs, B. M. and Amann, R., "Bacterioplankton compositions of lakes and oceans: a first comparison based on fluorescence in situ hybridization," Appl. Environ. Microbiol., 65(8), 3721-3726(1999).
7 Jang, A., Bishop, P. L., Okabe, S., Lee, S. G. and Kim, I. S., "Effect of dissolved oxygen concentration on the biofilm and in situ analysis by fluorescence in situ hybridization (FISH) and microelectrodes," Water Sci. Technol., 47(1), 49-57(2002).
8 Kim, G. T., Webster, G., Wimpenny, J. W., Kim, B. H., Kim, H. J. and Weightman, A. J., "Bacterial community structure, compartmentalization and activity in a microbial fuel cell," J. Appl. Microbiol., 101(3), 698-710(2006).   DOI   ScienceOn
9 Wagner, M., Amann, R., Lemmer, H. and Scheleifer, K., "Probing activated sludge with oligonucleotides specific for proteobacteria: inadequacy of culture-dependent methods for describing microbial community structure," Appl. Environ. Microbiol., 59(5), 1520-1525(1993).
10 Lazarova, V., Bellahcen, D., Manem, J., Stahl, D. A. and Rittmann, B. E., "Influence of operating conditions on population dynamics in nitrifying biofilms," Water Sci. Technol., 39(7), 5-11(1999).   DOI   ScienceOn
11 Kloep, F., Roske, I. and Neu, T. R., "Performance and microbial structure of a nitrifying fluidized-bed reactor," Water Res., 34(1), 311-319(2000).   DOI   ScienceOn
12 Szpyrkowicz, L., Rigoni-Stern, S. and Zilio, F., "Pilot plant studies on tannery wastwater treatment with the objective to reduce sludge production," Water Sci. Technol., 23, 1863-1871(1991).
13 Wagner, M., Erhart, R., Manz, W., Amann, R., Lemmer, H., Wedi, D. and Schleifer, K. H., "Development of an rRNAtargeted oligonucleotide probe specific for the genus acinetobacter and its application for in situ monitoring in activated sludge," Appl. Environ. Microbial., 60(3), 792-800(1994).
14 Mobarry, B. K., Wagner, M., Urbain, V., Rittmann, B. E. and Stahl, D. A., "Phylogenetic probes for analyzing abundance and spatial organization of nitrifying bacteria," Appl. Environ. Microbiol., 62(6), 2156-2162(1996).
15 Liu, W. T., Nielsen, A. T., Wu, J. H., Tsai, C. S., Matsuo, Y. and Molin, S., "In situ identification of polyphosphateand polyhydroxyalkanoate-accumulating traits for microbial populations in a biological phosphorus removal process," Environ. Microbiol., 3(2), 110-122(2001).   DOI   ScienceOn
16 Snaidr, J., Amann, R., Huber, I., Ludwig, W. and Schleifer, K. H., "Phylogenetic analysis and in situ identification of bacteria in activated sludge," Appl. Environ. Microbiol., 63(7), 2884-2896(1997).
17 Schramm, A., Beer, D., Wagner, M. and Amann, R., "Identification and activities in situ of Nitrosospira and Nitrospira spp. as dominant populations in a nitrifying fluidized bed reactor," Appl. Environ. Microbiol., 64(9), 3480-3485 (1998).
18 Mino, T., Satoh, H., Onuki, M., Akiyama, T., Nomura, T. and Matsuo, T., "Strategic approach for characterization of bacterial community in enhanced biological phosphate removal (EBPR) process," In Advances in Water and Wastewater Treatment Technology: Molecular Technology, Nutrient Removal, Sludge Reduction and Environmental Health, Matsuo, T., Hanaki, K., Takizawa, S., and Satoh, H., (Eds.), Elsevier, London(2001).
19 Nielsen, P. H., Kragelund, C., Seviour, R. J. and Nielsen, J. L., "Identity and ecophysiology of filamentous bacteria in activated sludge," FEMS Microbiol. Rev., 33, 969-998(2009).   DOI   ScienceOn
20 Wong, M. T., Mino, T., Seviour, R. J., Onuki, M. and Liu, W. T., "In situ identification and characterization of the microbial community structure of full-scale enhanced biological phosphorous removal plants in Japan," Water Res., 39, 2901-2914(2005).   DOI   ScienceOn
21 Araya, R., Tani, K., Tagaki, T., Yamaguchi, N. and Nasu, M., "Bacterial activity and community composition in stream water and biofilm from an urban river determined by fluorescent in situ hybridization and DGGE analysis," FEMS Microbiol. Ecol., 43, 111-119(2003).   DOI   ScienceOn
22 Patil, S. S., Kumar, M. S. and Ball, A. S., "Microbial community dynamics in anaerobic bioreactors and algal tanks treating piggery wastewater," Appl. Microbiol. Biotechnol., 87, 353-363(2010).   DOI   ScienceOn
23 Eikelboom, D. H., "Filamentous organisms observed in activated sludge," Water Res., 9, 365-388(1975).   DOI   ScienceOn
24 Richard, M., Hao, O. and Jenkins, D., "Growth kinetics of Sphaerotilus species and their significance in activated sludge bulking," J. Water Pollut. Control Fed., 57, 68-81(1985).
25 Andreasen, K. and Nielsen, P. H., "Application of microautoradiography to the study of substrate uptake by filamentous microorganisms in activated sludge," Appl. Environ. Microbiol., 63, 3662-3668(1997).
26 Zhang, T. C. and Bishop, P. L., "Evaluation of substrate and pH effects in a nitrifying biofilm," Water Environ. Res., 68(7), 1107-1115(1996).   DOI   ScienceOn
27 Eikelboom, D. H., Process Control of Activated Sludge Plants by Microscopic Investigation, IWA Publishing, London( 2000).