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Oxidative Coupling Reaction of Purified Aldrich Humic Acid by Horseradish Peroxidase  

Jee, Sang-Hyun (Department of Civil Engineering, Kyung Hee University)
Kim, Do-Gun (Department of Civil and Environmental Engineering, Korea Advanced Institute of Science and Technology)
Kim, Jeong-Hyun (Department of Civil Engineering, Kyung Hee University)
Ko, Seok-Oh (Department of Civil Engineering, Kyung Hee University)
Publication Information
Journal of Korean Society of Environmental Engineers / v.32, no.11, 2010 , pp. 1054-1062 More about this Journal
Abstract
Oxidative coupling reactions of humic substances (HS) can be catalyzed by a variety of natural extracellular enzymes and metal oxides. In this study, property changes of HS induced by a natural enzyme, horseradish peroxidase (HRP), and the effect of it to microfiltration (MF) were investigated. PAHA was transformed by oxidative coupling reaction with HRP and hydrogen peroxide ($H_2O_2$), verifying the catalytic effects of the HRP. Size exclusion chromatography (SEC) revealed that weight-average molecular weight (MWw) of PAHA was proportionally increased with the dosages of HRP and $H_2O_2$, indicating the transform action of HS into larger and complex molecules. An increase in the conformational stability of HS was achieved through the promotion of intermolecular covalent bondings between heterogeneous humic molecules. Spectroscopic analysis (fluorescence and infrared spectroscopy) proved that functional groups were transformed by the reaction. Additionally, HS and transformed products were undergone microfiltration (MF) to examine the treatment potential of them in a water treatment facility. Original HS could not be removed by MF but larger molecules of transformed products could be removed. Meanwhile, transformed products caused more fouling on the filtration than original HS. This results proved that natural organic matter (NOM) can be removed by MF after its increase in molecular size by oxidative coupling reaction.
Keywords
Humic Acid; Oxidation Coupling Reaction; Horseradish peroxidase (HRP); Hydrogen Peroxide ($H_2O_2$); Catalytic Effect;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 Huang, Q. and Weber, Jr. W. J., "Peroxidase-catalyzed coupling of phenol in the presence of model inorgarnic and organic solid phases," Environ. Sci. Technol., 38, 5238-5245(2004).   DOI   ScienceOn
2 Zarruk, K. K., Scholer, G. and Dudal, T., "Fluorescence fingerprints and $Cu^{2+}$-complexing ability of individual molecular size fractions in soil-and waste-borne DOM," Chemosphere, 69, 540-548(2007).   DOI   ScienceOn
3 Chen, W., P. Westerhoff, J. A. Leenheer and Booksh, K., "Fluorescence citation-emission matrix regional integration to quantify spectra for dissolved organic matter," Environ. Sci. Technol., 37, 5701-5710(2003).   DOI   ScienceOn
4 Huang, Q., Pinto, R. A., Burlingame, D. J., Tang, J. and Weber, Jr. W. J., "Enhanced removal of natural organic matter via peroxidase-mediated oxidative coupling," Water Sci. Technol.; Water Supply, 4(4), 33-40(2004).
5 Piccolo, A., Cozzolino, A., Conte, P. and Spaccini, R., "Polymerization of humic substances by an enzyme-catalyzed oxidative coupling," Naturwissenschaften, 87, 391-394(2000).   DOI   ScienceOn
6 Wang, Z., Wu, Z. and Tang, S., "Characterization of dissolved organic matter in a submerged membrane bioreactor by using three-dimensional excitation and emission matrix fluorescence spectroscopy," Water Res., 43, 1533-1540(2009).   DOI   ScienceOn
7 Uyguner, C. S. and Bekbolet, M., "Evaluation of humic acid photocatalytic degradation by UV-vis and fluorescence spectroscopy," Catal. Today, 101, 267-274(2005).   DOI   ScienceOn
8 Dec, J., Haider, K. and Bollag, J-M., "Release of substituents from phenolic compounds during oxidative coupling reactions," Chemosphere, 52, 549-556(2003).   DOI   ScienceOn
9 Stevenson, F. J., Humus chemistry, John Wiley & Sons, Inc., New York(1994).
10 Wang, M. C. and Huang, P. M., "Ring cleavage and oxidative transformation of pyrogallol catalyzed by Mn, Fe, Al, and Si oxides," Soil Sci., 165(12), 934-942(2000).   DOI   ScienceOn
11 Piccolo, A., Conte, P. and Tagliatesta, P., "Increased conformational rigidity of humic substances by oxidative biomimetic catalysis," Biomacromolecules, 6, 351-358(2005).   DOI   ScienceOn
12 Smejkalova, D., P. Conte and Piccolo, A., "Structural characterization of isomeric dimers from the oxidative oligomerization of catechol with a biomimetic catalyst," Biomacromolecules, 8, 737-743(2007).   DOI   ScienceOn
13 Bollag, J.-M., "Decontaminating soil with enzymes," Environ. Sci. Technol., 26, 1876-1881(1992).   DOI
14 Shindo, H. and Huang, P. M., "Catalytic effects of manganese (IV), iron (III), aluminum, and silicon oxides on the formation of phenolic polymers," Soil Sci. Soc. Am. J., 48, 927-934(1984).   DOI   ScienceOn
15 Matocha, C. J., Sparks, D. L., Amonette, J. E. and Kukkadapu, R. K., "Kinetics and mechanism of birnessite reduction by catechol," Soil. Sci. Soc. Am. J., 65, 58-66(2001).   DOI   ScienceOn
16 Kilduff, J. and Weber, Jr. W. J., "Transport and separation of organic macromolecules in ultrafiltration processes," Environ. Sci. Technol., 26, 569-577(1992).   DOI
17 Kilduff, J. E., Karanfil, T., Chin, Y-P. and Weber, Jr. W. J., "Adsorption of natural organic polyelectrolytes by activated carbon: a size-exclusion chromatography study," Environ. Sci. Technol., 30, 1336-1343(1996).   DOI   ScienceOn
18 허진, 김미경, 박성원, "상류 오염원 추적을 위한 용존 유기물질 Sychronous 형광스펙트럼 분석 연구," 대한환경공학회지, 29(3), 317-324(2007).   과학기술학회마을
19 Hur, J. and Schlautman, M. A., "Molecular weight fractionation of humic substances by adsorption onto minerals," J. Colloid Interface Sci., 264, 313-321(2003).   DOI   ScienceOn