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Influence of Surfactants on Enhancing Transport of Bacteria in Geological Materials  

Choi, Nag-Choul (Environmental Biocolloid Engineering Laboratory, Seoul National University)
Park, Seong-Jik (Environmental Biocolloid Engineering Laboratory, Seoul National University)
Kim, Song-Bae (Environmental Biocolloid Engineering Laboratory, Seoul National University)
Kim, Dong-Ju (Environmental Hydrogeology Laboratory, Korea University)
Lee, Seong-Jae (Environmental R&D Center, Halla Energy & Environment)
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Abstract
This study investigated the effect of surfactants (nonionic surfactant (Tween 20), biosurfactant) on enhancing transport of bacteria (Bacillus subtilis ATCC 6633) in geological materials. Column experiments were performed under various surfactant conditions with columns packed with quartz sand (particle size distribution: 0.5~2.0 mm, mean diameter: 1.0 mm). Bacterial mass recovery, sticking efficiency, and other parameters were quantified from breakthrough curves. Results indicate that bacterial attachment to sand surfaces increased considerably in the presence of mineral salt medium (MSM), especially at the inlet, which was due to the increase of ionic strength by MSM. It was observed that bacterial transport in sand columns was enhanced in the presence of surfactant. Results also show that simultaneous injection of both surfactant and MSM or pre-injection of surfactant was more effective in bacterial transport enhancement than after-injection of surfactant. This study suggests that transport of bacteria in geological materials could be influenced by surfactants and their injection methods.
Keywords
Bacterial Transport; Nonionic Surfactant; Biosurfactant; Geological Materials;
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