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Characteristics of Antibiotic Resistant Bacteria in Urban Sewage and River  

Oh, Hyang-Kyun (School of Civil and Environmental Engineering, Yonsei University)
Park, Joon-Hong (School of Civil and Environmental Engineering, Yonsei University)
Publication Information
Journal of Korean Society of Environmental Engineers / v.31, no.3, 2009 , pp. 232-239 More about this Journal
Abstract
This research investigated the characteristics of antibiotic resistance of bacteria in microbial communities from municipal wastewater treatment plants (MWTPs), and monitored seasonal changes of antibiotic resistant bacteria (ARB) from MWTPs and Han river. When antibiotics were amended to either R2A agar (R2A) for general heterotrophs or MacConeky sorbitol agar (MSA) for coliform bacteria, all the MWTP samples exhibited multiple antibiotic resistance on the antibiotic-amended solid media. The antibiotic resistance appearing frequencies of ampicillin and sulfathiazole, respectively, were higher than reported data for other countries. The antibiotic resistance appearances differed depending upon the concentrations of primary substrate and nutrients and the types of cultivation media. The following 16S rRNA gene phylogenetic analysis showed that the identified multiple-antibiotic resistant microbes on R2A plates were more likely to be known human-pathogenic bacteria than the background heterotrophic bacteria were, suggesting a high risk of antibiotic resistance appearance to public health. In addition, according to our investigation of seasonal changes of ARB from urban MWTP and river samples, the frequency of ARB appearances was shown to correlate positively with temperature. This indicates a possibility that global warming result in increase in microbial risk to public health.
Keywords
Antibiotic Resistance; Microbial Risk; Water Quality; Urban Sewage;
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1 Baquero, F., Mart$\nez, J-L., and Cant$\n, R., “Antibiotics and antibiotic resistance in water environments,” Curr. Opin. Biotechnol., 19, 260-265(2008)   DOI   ScienceOn
2 Kahn, L. H., “The scourge of antibiotic-resistant bacteria,” Bull. At. Sci., 16th December(2007)
3 Iwane, T., Urase, T., and Yamamoto, K., “Possible impact of treated wastewater discharge on incidence of antibiotic resistant bacteria in river water,” Water Sci. Technol., 43(2), 91-99(2001)   PUBMED   ScienceOn
4 Mart$\nez, J. L., “Antibiotics and antibiotic resistance genes in natural environments,” Science, 321, 365-367(2008)   DOI   PUBMED   ScienceOn
5 Guardabassi, L., Danilo, M. A. L. F. W., and Dalsgaard, A., “The effects of tertiary wastewater treatment on the prevalence of antimicrobial resistant bacteria,” Water Res., 36, 1955-1964(2002)   DOI   ScienceOn
6 Middleton, J. H. and Ambrose, A., “Enumeration and antibiotic resistance patterns of fecal indicator organisms isolated from migratory Canada geese (Branta Canadensis),” J. Wildl. Dis., 41(2), 334-341(2005)   DOI   PUBMED   ScienceOn
7 Sambrook, J. and Russell, D. W., “Molecular Clonning : A Laboratory Manual, Cold Spring Harbor,” New York (2001)
8 Lane, D. J., “16S/23S rRNA sequencing. in: E. Stackebrandt and M. Goodfellow (Eds.), Nucleic acid techniques in bacterial systematics,” John Wiley and Sons, New York, NY, 115-175(1991)
9 Salyers, A. A., Gupta, A., and Wang, Y., “Human intestinal bacteria as reservoirs for antibiotic resistance genes,” Trends Microbiol., 12(9), 412-416(2004)   DOI   ScienceOn
10 Whitehead, T. R., Cotta, M. A., Whittle, G., Shoemaker, N., and Salyers, A. A., “The commensal bacterial populations of swine feces and stored swine manure: reservoirs of antibiotic resistance?,” J. Anim. Sci., 81, 461(2003)
11 Krcm$\ry, V., Bajizukov$\acute{a}$, A., Langs$\acute{a}$dl, L., Kotuliakov$\acute{a}$, M., and Sobotov$\acute{a}$, O., “Evaluation of the resistance of Enterobacterizceae strains to antibiotics-comparison of strains from clinical material versus environment,” J. Hyg. Epidemiol. Microbiol. Immunol., 33(3), 299-304(1989)   ScienceOn
12 Ash, R. J., Mauck, B., and Morgan, M., “Antibiotic resistance of gram-negative bacteria in rivers, United States,” Emerging Infect. Dis., 8(7), 713-716(2002)   DOI   PUBMED   ScienceOn
13 식품의약품안전청, “환경 중 항생제내성균 모니터링,” (2006)
14 Hance, F. L., Steingart, K. R., Hahn, C. G., Pascopella, L., and Nolan, C. M., “Field assessment of a model tuberculosis outbreak response plan for low-incidence areas,” BMC Public Health, 7, 307-314(2007)   DOI   PUBMED
15 Reasoner, D. J. and Geldreich, E. E., “A new medium for the enumeration and subculture of bacteria from potable water,” Appl. Environ. Microbiol., 49(1), 1-7(1985)   PUBMED   ScienceOn
16 Hu, Z., Shi, J., Chang, H., Li, D., Yang, M., and Kamagata, Y., “Phenotyping and genotyping of antibioticresistant Escherichia coli isolated from a natural river basin,” Environ. Sci. Technol., 42, 3415-3420(2008)   DOI   ScienceOn
17 http://www.ebi.ac.uk/clustalw
18 Chopra, I. and Roberts, M., “Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology of bacterial resistance,” Microbiol. Mol. Biol. Rev., 65(2), 232-260(2001)   DOI   ScienceOn
19 Tamanai-Shacoori, Z., Arturo, M., Pommepuy, M., Mamez, C., and Cormier, M., “Conjugal transfer of natural plasmids between E.coli strains in sterile environmental water,” Curr. Microbiol., 30, 155-160(1995)   DOI   ScienceOn
20 국립환경과학원, “환경 중 의약물질 분석방법 연구 및 노출실태 조사 보고서,” (2006)
21 Suzuki, M. T. and Giovannoni, S. J., “Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR,” Appl. Environ. Microbiol., 62(2), 625-630(1996)   PUBMED   ScienceOn
22 Sutherst, R. W., 'Global change and human vulnerability to vector-borne diseases,' Clin. Microbiol. Rev., 17(1), 136-173(2004)   DOI   PUBMED   ScienceOn
23 McCaig, A. E., Embely, T. M., and Prosser, J. I., “Molecular analysis of enrichment cultures of marine ammonia oxidisers,” FEMS Microbiol. Lett., 120, 363-368(1994)   DOI   ScienceOn
24 Lima-Bittencourt, C. I., Cursino, L., Gon$\c{c}$alves-Dornelas, H., Pontes, D. S., Nardi, R. M., Callisto, M., Chartone-Souza, E., and Nascimento, A. M., “Multiple antimicrobial resistance in Enterobacteriaceae isolates from pristine freshwater,” Genet. Mol. Res., 6(3), 510-521(2007)   PUBMED   ScienceOn
25 Daly, M. and Fanning, S., “Characterization and Chromosomal Mapping of Antimicrobial Resistance Genes in Salmonella enterica Serotype Typhimurium,” Appl. Environ. Microbiol., 66(11), 4842-4848(2000)   DOI   ScienceOn
26 Levy, S. B. and Marshall, B., “Antibacterial resistance worldwide: causes, challenges and responses,” Nat. Med., 10(12), S122-S129(2004)   DOI   ScienceOn
27 Huycke, M. M., Sahm, D. F., and Gilmore, M. S., “Multiple-drug resistant Enterococci: the nature of the problem and an agenda for the future,” Emerg Infect Dis., 4(2), 239-249(1998)   DOI   ScienceOn
28 http://www.macrogen.co.kr
29 Cook, M., Moloto, E., and Anerson, C., “Fluorochrome labelling in roman period skeletons from dakhleh oasis, Egypt,” Am. J. Phys. Anthropol., 80(2), 137-143(1989)   DOI   ScienceOn
30 환경부, 먹는 물 수질공정시험 방법(2007)
31 Boon, P. I. and Cattanach, M., “Antibiotic resistance of native and faecal bacteria isolated from rivers, reservoirs and sewage treatment facilities in Victoria, south-eastern Australia,” Lett. Appl. Microbiol., 28(3), 164-168(1999)   DOI   ScienceOn
32 Dantas, G., Sommer, M. O. A., Oluwasegun, R. D., and Church, G. M., “Bacteria subsisting on Antibiotics,” Science, 320, 100-103(2008)   DOI   PUBMED   ScienceOn
33 Moura, A., Henriques, I., Ribeiro, R., and Correia, A., “Prevalence and characterization of integrons from bacteria isolated from a slaughterhouse wastewater treatment plant,” J. Antimicrob. Chemother, 60(6), 1243-1250(2007)   DOI   ScienceOn
34 Kim, S., Jensen, J. N., Aga, D. S., and Weber, A. S., “Tetracycline as a selector for resistant bacteria in activated sludge,” Chemosphere, 66, 1643-1651(2007)   DOI   ScienceOn