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Bioluminescence Activity of Toluene Analogs by Alginate-immobilized Pseudomonas putida mt-2 KG1206  

Kong, In-Chul (Department of Environmental Engineerting, Yeungnam University)
Jung, Hong-Kyung (Department of Environmental Engineerting, Yeungnam University)
Ko, Kyung-Seok (Geologic Environment Division, Korea Institute of Geoscience & Mineral Resources (KIGAM))
Publication Information
Journal of Korean Society of Environmental Engineers / v.31, no.2, 2009 , pp. 147-152 More about this Journal
Abstract
In this study, the applicability of alginate-immobilized Pseudomonas putida mt-2 KG1206 on the environments, contaminated with toluene analogs was conducted. Genetically engineered strain KG1206 produces light by direct (m-toluate, benzoate) and indirect (toluene, xylenes) inducers. The protocol for the alginate-immobilization was determined in terms of the cell to alginate ratio, solution, proper number of alginate beads, and other conditions. Maximum bioluminescences of five chemicals by immobilized strain were generally observed in following orders: m-toluate > p-xylene > toluene > o-xylene > m-xylene. In relationship between bioluminescence activity and inducer reduction, initial m-toluate (5 mM) in solution was removed approximately 48% of initial at 5 h exposure, showing continuous decrease of inducer chemical in solution. These results of study with alginate-immobilized beads would be useful, especially, for biomonitoring of contaminated environments with specific compounds, such as petroleum hydrocarbon compounds including toluene analogs.
Keywords
Alginate; Bioluminescence; Genetically Engineered Strain; Immobilization; Toluene Analogs;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Shim, H. J., Shin, E. B., and Yang, S. T., “A continuous fibrous-bed bioreactor for BTEX biodegradation by a co-culture of Pseudomonas putida and Pseudomonas fluorescens,” Adv. Environ. Res., 7, 203-216(2002)   DOI   ScienceOn
2 Willardson, B. M., Wilkins, J. F., Rand, T. A., Schupp, J. M., Hill, K. K., Keim, P., and Jackson, P. J., “Development and testing of a bacterial biosenseor for toluenebased environmental contaminants,” Appl. Environ. Microbiol., 64(3), 1006-1012(1998)   PUBMED   ScienceOn
3 Kong, I. C., Park, T. H. and Ahn, T. B., “Bioluminescence activity and degradation kinetics of genetically engineered strain P. putida mt-2 RB1401 in soil system with m-xylene,” Soil. Sediment Contam., 14, 135-142(2005)   DOI   ScienceOn
4 Kong, I. C., Kim, M., Ko, K. S., Kim, J. G., Jeon, C. W., and Bhandari, A., “Use of recombinant bioluminescence bacteria for on-site monitoring of toluene analogs at petroleum contaminated sites,” J. Environ. Eng., 133(7), 772-776(2007)   DOI   ScienceOn
5 공인철, 서혜영, 이건우, 김승현, 백성옥, “토양에 오염된 휘발성 유기화합물의 모니터링을 위한 발광 유전자 재조합 균주 이용 가능성,” 대한환경공학회지, 25(4), 440-445(2003)   과학기술학회마을   ScienceOn
6 Assinder, S. J. and Willams, P. A., “The TOL Plasmid: Determinants of the catabolism of toluene and the xylenes,” Adv. Microbiol. Phys., 31, 2-69(1990)   PUBMED
7 Nealson, K. H. and Hastings, J. W., “Bacterial bioluminescence: Its control and ecological significance,” Microbiol. Rev., December, 496-518(1979)   PUBMED   ScienceOn
8 Xi, W. W. and Xu, J. H., “Preparation of enantiopure(S)-ketoprofen by immobilized Candida rugosa lipase in packed bed reactor,” Process Biochem., 40(6), 2161-2166(2005)   DOI   ScienceOn
9 Cheetham, P. S. J. and Bucke, C., “Immobilizatin of microbial cells and their use in waste water treatment,” Microbiological Methods for Environmental Biotechnology, Grainger, J. M. and Lynch, J. M.(Eds.), Academic Press Inc, London, UK, pp. 219-235(1984)
10 Hotel, A., Marques, S., Mohler, I., Jakubzik, U., and Timmis, K. N., “Carbon source-dependent inhibition of xyl operon expression of the Pseudomonas putida TOL plasmid,” J. Bacteriol., 176(6), 1773-1776(1994)   DOI
11 하상안, 염혜경, “저온 열 탈착에 의한 유류 오염토의 처리 조건의 연구,” 대한환경공학회지, 29(8), 956-960(2007)   과학기술학회마을   ScienceOn
12 이상민, 배희경, “재조합 발광대장균과 해양 발광 미생물을 이용한 중금속 급성독성평가,” 대한환경공학회지, 27(8), 900-906(2005)   과학기술학회마을
13 Li, Y. F., Li, F., Y., Ho, C. L., and Liao, V. H. C., “Construction and comparison of fluorescence and bioluminescence bacterial biosensors for the detection of bioavailable toluene and related compounds,” Env. Pollut., 152(1), 123-129(2008)   DOI   ScienceOn