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Change of Microbial Community and Fermentative Production of Hydrogen from Tofu Wastewater  

Jun, Yoon-Sun (Department of Environmental Engineering, Seoul National University of Technology)
Joe, Yoon-A (Department of Environmental Engineering, Seoul National University of Technology)
Lee, Tae-Jin (Department of Environmental Engineering, Seoul National University of Technology)
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Abstract
In this study, characteristics of biological hydrogen production and microbial distribution were investigated with the wastewater of Tofu manufacturing process. Comparison of hydrogen production was conducted with acid or base pre-treatment of the wastewater. Maximum hydrogen production was acquired with combination of heat and acid treatment. Hydrogen production ($P_h$) and maximum hydrogen production rate ($R_h$) was calculated 661.01 mL and 12.21 mL/g dry wt biomass/hr from the modified Gompartz equation. Most of microbial community was analyzed as Streptococcus sp. from PCR-DGGE experiment of 16S rDNA. It was concluded that most significant microorganism for hydrogen production was Streptococcus gallolyticus sub sp. in this experiment.
Keywords
Anaerobic Fermentation; Hydrogen Production; Streptococcus; PCR-DGGE; Tofu Wastewater;
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