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Effect of Bioluminescence Stimulating Agent of the Genetically Engineered Strain KG1206 on the Monitoring of the Petroleum Hydrocarbon Contaminated Groundwater Samples  

Ko, Kyung-Seok (Geological & Environmental Hazards Division, Korea Institute of Geoscience & Mineral Resources(KIGAM))
Kong, In-Chul (School of Construction and Environmental Engineering, Yeungnam University)
Publication Information
Journal of Korean Society of Environmental Engineers / v.30, no.1, 2008 , pp. 79-84 More about this Journal
Abstract
This paper describes the application of bioluminescence stimulating agents on a genetically engineered microorganism, Pseudomonas putida mt-2 KG1206, to monitor toluene analogs using in groundwater samples from petroleum hydrocarbon contaminated sites. The maximum bioluminescent response with pure chemicals followed in the order: m-methyl benzyl alchohol > m-toluate > toluene > m-xylene > benzoate > p-xylene > o-xylene. Generally, the bioluminescence production of strain mixed with groundwater samples was dependent on the contaminated total inducer concentrations. However, few samples showed opposite results, where these phenomena may be caused by the complexicity of environmental samples. Two chemicals, SL(sodium lactate) and KNO$_3$, were tested to determine a better bioluminescence stimulant. Both chemicals stimulate the bioluminescence activity of strain KG1206, however, a slightly high bioluminescence was observed with nitrogen chemical. This selected stimulant was then tested on samples collected from contaminated groundwater samples. The bioluminescence activity of all samples mixed with the strain was stimulated with KNO$_3$ amendment. This suggests that the low bioluminescence activity exhibited by the environmental groundwater samples can be stimulated by amending the culture with a proper agent, such as nitrogen compound. These findings would be useful, especially, when strain was used to monitor the groundwater samples contaminated with low inducer contaminants. Overall, the results of this study found the ability of bioluminescence producing bacteria to biosensor a specific group of environmental contaminants, and suggest the potential for more efficient preliminary application of this engineered strain in a field-ready bioassay.
Keywords
Bioluminescence; Genetically Engineered Bacteria; Groundwater; Stimulant; Toluene Analogs;
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