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BTEX Biodegradation in Contaminated Soil Samples Using Pure Isolates and Changes in the Mixed Microbial Community Structure  

Chung, Kyung-Mi (Water Environment and Remediation Center, Korea Institute of Science and Technology)
Choi, Yong-Su (Water Environment and Remediation Center, Korea Institute of Science and Technology)
Hong, Seok-Won (Water Environment and Remediation Center, Korea Institute of Science and Technology)
Lee, Soo-Jin (Water Environment and Remediation Center, Korea Institute of Science and Technology)
Lee, Sang-Hyup (Water Environment and Remediation Center, Korea Institute of Science and Technology)
Publication Information
Journal of Korean Society of Environmental Engineers / v.28, no.7, 2006 , pp. 757-763 More about this Journal
Abstract
In our previous studies, we have isolated bacteria from BTEX-contaminated sediment, which utilized BTEX as a sole carbon source and $NO_3$-N as an electron acceptor. For the possibility of field application, we have applied co-culture of those isolates in the BTEX-contaminated soil and evaluated their biodegradation efficiencies. To investigate the relationship between the isolates and indigenous microorganism in soil, changes of microbial community structure in soil samples with respect to time were monitored. To examine this, soil samples were artificially contaminated with benzene, toluene, ethylbenzene and o-xylene. BTEX-degrading bacteria such as Pseudomonas stutzeri strain 15(DQ 202712), Klebsiells sp. strain 20(DQ 202715) and Citrobacter sp. strain A(DQ 202713) were injected into the soil samples in the ratio of 2:1:1. Our results showed that the highest BTEX biodegradation efficiency was achieved when both BTEX and $NO_3-N$ existed simultaneously. The change in soil microbial community structure was characterized by PCR-DGGE analysis comparing the relative DGGE band intensities. The band intensities of indigenous microorganisms in the soil were reduced by injecting co-culture of the three isolates. On the contrary, the relative band intensities of the isolates were increased. Among the three isolates, Pseudomonas stutzeri strain 15 rendered the highest band intensity. This indicates that the Pseudomonas stutzeri was the dominant microbial species found in the soil samples.
Keywords
Biodegradation; BTEX; DGGE; Microbial Community; Pseudomonas stutzeri;
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Times Cited By KSCI : 1  (Citation Analysis)
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