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Characterization of Denitrifier Community in Independent Anoxic Reactor Using Modified BAF Process  

Park, Jeung-Jin (Department of Environmental Engineering, Pusan National University)
Jeung, Young-Rok (Department of Environmental Engineering, Pusan National University)
Yu, Jae-Cheul (Department of Environmental Engineering, Pusan National University)
Hur, Sung-Ho (Department of Environmental Engineering, Pusan National University)
Choi, Won-Seok (Bioentech company, Ltd.)
Byun, Im-Gyu (Institute for Environmental Technology and Industry, Pusan National University)
Lee, Tae-Ho (Department of Environmental Engineering, Andong National University)
Park, Tae-Joo (Department of Environmental Engineering, Pusan National University)
Publication Information
Journal of Korean Society of Environmental Engineers / v.28, no.7, 2006 , pp. 752-756 More about this Journal
Abstract
The independent anoxic reactor was introduced in biological aerated filters as the regulation of water quality requirement, especially total nitrogen, had been strengthened. The process studied in this work was upflow $Biobead^{(R)}$ process which was used commercial invented for removal of organic materials and nitrification. For the purpose of evaluating the independent anoxic reactor, PCR-DGGE, of the molecular biological methods, was performed. Two types of nitrite reductase genes were selected. One is nirS represented cytocrome $cd_1$ nitrite reductase gene and the other is nirK represented Cu-containing nitrite reductase gene. Denitrifier community in the independent anoxic reactor was analyzed with PCR-DGGE using these two denitrifying functional genes. As the result of the PCR, only nirS gene was detected between nirS and nirK. With the result of the DGGE, specific bands became strong, as the operating days were longer, nitrate loading rate was increased. otherwise those of the initial activated sludge showed various bands. In the consequence of the sequence of DGGE bands, various denitrifiers were sequenced in the initial activated sludge, while specific denitrifiers like alcaligenes faecalis were predominant in the anoxic reactor. Consequently, introduction of the independent anoxic reactor made it possible to achieve 96% denitrification efficiency, and was proper for the modification of BAF process.
Keywords
Denitrification; PCR; DGGE; Biological Aerated Filter(BAF); Microbial Community;
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