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Estimation of Estrogenic Activity by E-screen Assay and Stepwise Analysis of Endocrine Disruptors in Pilot Sewage Treatment Plant  

Lee, Ji-Ho (Gwangju Institute of Science and Technology, Department of Environmental Science and Engineering)
Park, Jong-Yol (Gwangju Institute of Science and Technology, Department of Environmental Science and Engineering)
Ra, Jin-Sung (Gwangju Institute of Science and Technology, Department of Environmental Science and Engineering)
Duong, Cuong N. (Gwangju Institute of Science and Technology, Department of Environmental Science and Engineering)
Lee, Byoung-Cheun (Gwangju Institute of Science and Technology, Department of Environmental Science and Engineering)
Kim, Sang-Don (Gwangju Institute of Science and Technology, Department of Environmental Science and Engineering)
Publication Information
Journal of Korean Society of Environmental Engineers / v.28, no.7, 2006 , pp. 697-703 More about this Journal
Abstract
Endocrine disruptors were measured with GC/MS in effluents discharged from sewage treatment processes in pilot scale for the purpose of water reuse. From that analysis, we compared the removal rate of them by treatment processes. Nonylphenol was mainly detected in effluents and high concentration from 0.36 to 0.94 ${\mu}g/L$. $17{\beta}$-estradiol(E2) and $17{\alpha}$-ethynylestradiol(EE2) were detected as below the limit of detection in effluent. Endocrine disruptors were removed effectively in the range from 50 to 100% by treatment process. EC50 value($9.0{\times}10^{-3}$ M) of $17{\beta}$-estradiol(E2) by dose response curve of E-screen assay has higher than that of bisphenol A($2.736{\times}10^{-5}M$) and p-octylphenol($9.760{\times}10^{-6}$ M). These results showed that alkylphenols have lower relative estrogen potency than other estrogens such as $17{\beta}$-estradiol(E2). Calculated estrogenic activity(ng-EEQ/L) was 2 times higher than measured total estrogenic activity which estimated by E-screen assay. Moreover estrogenic activity of effluent by treatment process showed very low as below 1 ng-EEQ/L.
Keywords
Endocrine Disruptors; Pilot Sewage Treatment Plant; E-screen Assay; Estrogenic Activity;
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