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Trace-level Determination of N-nitrosodimethylamine(NDMA) in Water Samples using a High-Performance Liquid Chromatography with Fluorescence Derivatization  

Cha, Woo-Suk (Center for Water R(CWR), Gwangju Institute of Science and Technology(GIST))
Fox, Peter (Department of Civil and Environmental Engineering, Arizona State University)
Nalinakumari, Brijesh (Department of Civil and Environmental Engineering, Arizona State University)
Choi, Hee-Chul (Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology(GIST))
Publication Information
Journal of Korean Society of Environmental Engineers / v.28, no.2, 2006 , pp. 223-228 More about this Journal
Abstract
High-performance liquid chromatography(HPLC) and fluorescence derivatization were applied for a trace-level N-nitrosodimethylamine(NDMA) analysis of water samples. Fluorescence intensity was optimized with the excitation wavelength of 340 nm and the emission wavelength of 530 nm. pH adjustment after denitrosation was necessary to get a maximum intensity at pH between 9 and 12. Maximum intensity was found with a dansyl chloride concentration of 330 to 500 mg/L. Percentile error in the water sample analyses through solid phase extraction was 12-162% and 6-23% for the lower concentration level(10-200 ng/L NDMA) and the higher level(100-1000 ng/L NDMA), respectively, showing more discrepancy in lower level. However, the average ratios of estimated NDMA to the standard NDMA were close to 1 for both concentration ranges, presenting this HPLC method could detect from tens to hundreds nanograms NDMA per liter. Accurate determination of NDMA, which was injected to a wastewater effluent, revealed the selectivity of fluorescence derivatization for the target compound(NDMA) in the presence of complex interfering compounds. The HPLC with fluorescence derivatization may be applicable for determining NDMA of water and wastewater samples fur various research purposes.
Keywords
N-nitrosodimethylamine(NDMA); HPLC; Fluorescence Derivatization; Dansyl Chloride; Water Reuse;
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