Browse > Article

Characterization of Signal Measuring System Using ion Selective Microelectrode and Electrometer  

Jun, Hyo-Yong (Department of Environmental Engineering, Pusan National University)
Seon, Kyeong-Suk (Department of Environmental Engineering, Pusan National University)
Park, Jeung-Jin (Department of Environmental Engineering, Pusan National University)
Byun, Im-Gyu (Institute for Environmental Technology and Industry, Pusan National University)
Park, Tae-Joo (Department of Environmental Engineering, Pusan National University)
Publication Information
Journal of Korean Society of Environmental Engineers / v.28, no.11, 2006 , pp. 1148-1153 More about this Journal
Abstract
Signal measuring system to analyze ion concentrations in biofilm was constructed with ion selective microeleclrode and electrometer. In order to evaluate the performance and applicability of signal measuring system, the following characteristics, such as slope of calibration curve, detection limit, variation of response according to the time, and potentiometric selectivity coefficient, were investigated. The slope of calibration curve showed high degree of association for primary ion concentration. The response of the system was log-linear in standard solution down to $10{\mu}M$ and signal measuring system was not sensitive for interfering ions. In comparison with commercial electrometer, the fabricated electrometer system had similar tendencies for the slope of calibration curve, detection limit, and response time. Therefore the signal measuring system could be used to investigate ion profiles in biofilm as a cost effective and reliable measuring system.
Keywords
Ion Selective Microelectrode; Electrometer; Biofilm; Ion Profile;
Citations & Related Records
연도 인용수 순위
  • Reference
1 van Loosdrecht, M. C. M., Tijhuis, L., Wijdieks, A. M. S., and Heijnen, J. J., 'Population distribution in aerobic biofilms on small suspended particles,' Water Sci. Technol., 31(1), 163 -171(1995)   DOI   ScienceOn
2 Richard P. Buck and Emo Linder, 'Recommendations for Nomenclature of Ion-selective Electrodes(IUPAC Recommendations 1994),' Pure & Appl. Chem., 68(12), 2527-2536( 1994)
3 De Beer, D., J. C. van den Heuvel, S. P. P. Ottengraf, 'Microelectrode Measurements of the Activity Distribution in Nitrifying Bacterial Aggregates,' Appl. Environ. Microbiol., 59(2), 573- 579(1992)
4 Okabe, S., Satoh, H., and Watanabe, Y., 'In situ analysis of nitrifying biofilms as determined by in situ hybridization and use of microelectrode,' Appl. Environ. Microbiol., 65(7), 3182 - 3191(1999)
5 장암, 이쌍구, 김성민, 김인수, '미소전극 센서를 이용 한 탈질 생물막내의 이온 농도측정,' 대한환경공학회지, 22(10), 1833 -1841(2000)
6 James. Halliwell, Michael Whitaker And David Ogden, 'Using microelectrode,' http://www.utdallas.edu/~tres/microelectrode/micro-lectrodes_ch01.pdf(2004)
7 김영오, 남해욱, 박태주, 'In Situ Hybridization에 의한 호기성 생물막내 질산화균의 성장 활성 및 분포특성과 미소전극에 의한 산소농도 분포,' 대한환경공학회지, 25(7), 803 - 809(2003)
8 Park, T. J., Nam, H. U., Kim, Y. O., Lee, J. H., and Kim, Y. G., 'Comparison of COD, nitrogen and phosphorous removal between anaerobic/anoxic/aerobic and anoxic/aerobic fixed biofilm reactor using SAC(synthetic activated ceramic) media,' Korean J. Chem. Eng., 15(4), 429-433(1998)   DOI
9 Lorenzen, J., Larsen, L. H., Kjaer, T., Revsbech, N. P., 'Biosensor Determination of the Microscale Distribution of Nitrate, Nitrate Assimilation, Nitrification, and Denitrification in a Diatom-Inhabited Freshwater Sediment,' Appl. Environ. Microbiol., 64(9), 3264 - 3269(1998)
10 De Beer, D., Schramm, A., Santegoted, C. M., and Kuhl, M., 'A nitrite microsenser for profiling enviromental biofilms,' Appl. Environ. Microbiol., 63(3), 973 -977 (1997)
11 Jang, A., Yoon, Y. H., Kim, In S., Kim, K. S., Bishop, P. L., 'Characterization and evaluation of aerobic granules in sequencing batch reactor,' Journal of Biotechnology, 105, 71 - 82(2003)   DOI   ScienceOn
12 A. J. Miller, 'Ion-Selective Microelectrodes for Measurement of Intracellular Ion Concentrations,' Methods in cell biology., 49, 275-291(1995)   DOI
13 이쌍구, 장암, 윤영한, 김인수, '이온선택성 미소전극의 특성 평가,' 대한환경공학회지, 25(2), 234 - 239(2002)
14 Liu, Y. and Capdeville, B., 'Specific activity of nitrifying biofilm in water nitrification process,' Water Res., 30(7), 1645 -1650(1996)   DOI   ScienceOn
15 Satoh, H., Ono, H., Rulin, B, Kamo, J., Fukushi, K. I., 'Macroscale and microscale analyses of nitrification and denitrification in biofilms attached on membrane aerated biofilm reactors,' Water Res., 38, 1633-1641(2004)   DOI   ScienceOn