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http://dx.doi.org/10.3795/KSME-A.2008.32.8.624

A Study About Biochip Combined with Micro Mixer and Reactor for DNA Ligation  

Kang, Do-Hyoung (한양대학교 대학원 기계공학과)
Ahn, Yoo-Min (한양대학교 기계공학과)
Hwang, Seung-Yong (한양대학교 분자생명과학부)
Publication Information
Transactions of the Korean Society of Mechanical Engineers A / v.32, no.8, 2008 , pp. 624-632 More about this Journal
Abstract
In this research, we developed new PDMS-glass based microbiochip consisted of the micromixer and microreactor for DNA ligation. The micromixer was composed of a straight channel integrated with nozzles and pillars, and the microreactor was composed of a serpentine channel. We coated the PDMS chip surface with the 0.25wt.% PVP solution to prevent the bubble generation which was caused by the hydrophobicity of the PDMS. The new micomixer was passive type and the mixing was enhanced by a convective diffusion using the nozzle and pillar. The 10.33mm long micromixer showed the good mixing efficiency of 87.7% at 500 l/min flow rate. We could perform the DNA ligation successfully in the microbiochip, and the ligation time was shortened from 4 hours in conventional laboratory method to 5 min in the microbiochip.
Keywords
Microbiochip; Micromixer; PVP Coating; DNA Ligation;
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Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By SCOPUS : 1
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